ABCA7 Monoclonal antibody, PBS Only

ABCA7 Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Pig

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2E1A10

Cat no : 67128-1-PBS

Synonyms

ABCA SSN, ABCA7, ABCX, Autoantigen SS N, FLJ40025, Macrophage ABC transporter

---

Validation Data Gallery

• 

  WB analysis of rat brain using 67128-1-Ig (same clone as 67128-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 67128-1-Ig (ABCA7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67128-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 67128-1-Ig (same clone as 67128-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 67128-1-Ig (ABCA7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67128-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 67128-1-Ig (same clone as 67128-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67128-1-Ig (ABCA7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67128-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig thymus using 67128-1-Ig (same clone as 67128-1-PBS)

  pig thymus tissue were subjected to SDS PAGE followed by western blot with 67128-1-Ig (ABCA7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67128-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig brain using 67128-1-Ig (same clone as 67128-1-PBS)

  pig brain tissue were subjected to SDS PAGE followed by western blot with 67128-1-Ig (ABCA7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67128-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human thymus using 67128-1-Ig (same clone as 67128-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human thymus tissue slide using 67128-1-Ig (ABCA7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67128-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human thymus using 67128-1-Ig (same clone as 67128-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human thymus tissue slide using 67128-1-Ig (ABCA7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67128-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human tonsillitis using 67128-1-Ig (same clone as 67128-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using ABCA7 antibody (67128-1-Ig, Clone: 2E1A10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67128-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human tonsillitis using 67128-1-Ig (same clone as 67128-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using ABCA7 antibody (67128-1-Ig, Clone: 2E1A10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67128-1-PBS in a different storage buffer formulation.

---

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67128-1-PBS targets ABCA7 in WB, IF, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

• Tested Reactivity: Human, Mouse, Rat, Pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: ABCA7 fusion protein Ag27499
• Full Name: ATP-binding cassette, sub-family A (ABC1), member 7
• Calculated Molecular Weight: 234 kDa
• Observed Molecular Weight: 250 kDa
• GenBank Accession Number: NM_019112
• Gene Symbol: ABCA7
• Gene ID (NCBI): 10347
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -80°C.