ABCA7 Monoclonal antibody

ABCA7 Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

2E1A10

Cat no : 67128-1-Ig

Synonyms

Autoantigen SS-N, Autoantigen SS N, ATP-binding cassette sub-family A member 7, ABCX, ABCA-SSN

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Validation Data Gallery

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  WB analysis using 67128-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67128-1-Ig (ABCA7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of pig brain using 67128-1-Ig

  pig brain tissue were subjected to SDS PAGE followed by western blot with 67128-1-Ig (ABCA7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 67128-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67128-1-Ig (ABCA7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of pig thymus using 67128-1-Ig

  pig thymus tissue were subjected to SDS PAGE followed by western blot with 67128-1-Ig (ABCA7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig brain using 67128-1-Ig

  pig brain tissue were subjected to SDS PAGE followed by western blot with 67128-1-Ig (ABCA7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 67128-1-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 67128-1-Ig (ABCA7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human thymus using 67128-1-Ig

  Immunohistochemical analysis of paraffin-embedded human thymus tissue slide using 67128-1-Ig (ABCA7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human thymus using 67128-1-Ig

  Immunohistochemical analysis of paraffin-embedded human thymus tissue slide using 67128-1-Ig (ABCA7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human tonsillitis using 67128-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using ABCA7 antibody (67128-1-Ig, Clone: 2E1A10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human tonsillitis using 67128-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using ABCA7 antibody (67128-1-Ig, Clone: 2E1A10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: 37°C incubated rat brain tissue, Jurkat cells, mouse brain tissue, pig brain tissue, pig thymus tissue, unboiled mouse brain tissue, unboiled rat brain tissue
• Positive IHC detected in: human thymus tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: human tonsillitis tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67128-1-Ig targets ABCA7 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: ABCA7 fusion protein Ag27499
• Full Name: ATP-binding cassette, sub-family A (ABC1), member 7
• Calculated Molecular Weight: 234 kDa
• Observed Molecular Weight: 250 kDa
• GenBank Accession Number: NM_019112
• Gene Symbol: ABCA7
• Gene ID (NCBI): 10347
• RRID: AB_2882427
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.