Various lysates were subjected to SDS PAGE followed by western blot with 67128-1-Ig (ABCA7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 67128-1-Ig (ABCA7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of pig brain using 67128-1-Ig
pig brain tissue were subjected to SDS PAGE followed by western blot with 67128-1-Ig (ABCA7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
pig brain tissue were subjected to SDS PAGE followed by western blot with 67128-1-Ig (ABCA7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 67128-1-Ig
Jurkat cells were subjected to SDS PAGE followed by western blot with 67128-1-Ig (ABCA7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
Jurkat cells were subjected to SDS PAGE followed by western blot with 67128-1-Ig (ABCA7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of pig thymus using 67128-1-Ig
pig thymus tissue were subjected to SDS PAGE followed by western blot with 67128-1-Ig (ABCA7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
pig thymus tissue were subjected to SDS PAGE followed by western blot with 67128-1-Ig (ABCA7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of pig brain using 67128-1-Ig
pig brain tissue were subjected to SDS PAGE followed by western blot with 67128-1-Ig (ABCA7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
pig brain tissue were subjected to SDS PAGE followed by western blot with 67128-1-Ig (ABCA7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 67128-1-Ig
mouse brain tissue were subjected to SDS PAGE followed by western blot with 67128-1-Ig (ABCA7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
mouse brain tissue were subjected to SDS PAGE followed by western blot with 67128-1-Ig (ABCA7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
IHC staining of human thymus using 67128-1-Ig
Immunohistochemical analysis of paraffin-embedded human thymus tissue slide using 67128-1-Ig (ABCA7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human thymus tissue slide using 67128-1-Ig (ABCA7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human thymus using 67128-1-Ig
Immunohistochemical analysis of paraffin-embedded human thymus tissue slide using 67128-1-Ig (ABCA7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human thymus tissue slide using 67128-1-Ig (ABCA7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of human tonsillitis using 67128-1-Ig
Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using ABCA7 antibody (67128-1-Ig, Clone: 2E1A10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using ABCA7 antibody (67128-1-Ig, Clone: 2E1A10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
IF Staining of human tonsillitis using 67128-1-Ig
Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using ABCA7 antibody (67128-1-Ig, Clone: 2E1A10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using ABCA7 antibody (67128-1-Ig, Clone: 2E1A10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
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human thymus tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected in
human tonsillitis tissue
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:5000-1:50000
Immunohistochemistry (IHC)
IHC : 1:50-1:500
Immunofluorescence (IF)-P
IF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Product Information
67128-1-Ig targets ABCA7 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
Various lysates were subjected to SDS PAGE followed by western blot with 67128-1-Ig (ABCA7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of pig brain using 67128-1-Ig
pig brain tissue were subjected to SDS PAGE followed by western blot with 67128-1-Ig (ABCA7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 67128-1-Ig
Jurkat cells were subjected to SDS PAGE followed by western blot with 67128-1-Ig (ABCA7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of pig thymus using 67128-1-Ig
pig thymus tissue were subjected to SDS PAGE followed by western blot with 67128-1-Ig (ABCA7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of pig brain using 67128-1-Ig
pig brain tissue were subjected to SDS PAGE followed by western blot with 67128-1-Ig (ABCA7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 67128-1-Ig
mouse brain tissue were subjected to SDS PAGE followed by western blot with 67128-1-Ig (ABCA7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of human thymus using 67128-1-Ig
Immunohistochemical analysis of paraffin-embedded human thymus tissue slide using 67128-1-Ig (ABCA7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human thymus using 67128-1-Ig
Immunohistochemical analysis of paraffin-embedded human thymus tissue slide using 67128-1-Ig (ABCA7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF-P Figures
IF Staining of human tonsillitis using 67128-1-Ig
Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using ABCA7 antibody (67128-1-Ig, Clone: 2E1A10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
IF Staining of human tonsillitis using 67128-1-Ig
Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using ABCA7 antibody (67128-1-Ig, Clone: 2E1A10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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