4EBP1 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

4EBP1 Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, rat, pig, mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2C3F3

Cat no : 60246-1-PBS

Synonyms

4E BP1, 4EBP1, BP 1, eIF4E binding protein 1, EIF4EBP1, PHAS I

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Validation Data Gallery

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  WB analysis of K562 using 60246-1-Ig (same clone as 60246-1-PBS)

  WB result of 4EBP1 antibody (60246-1-Ig, 1:4000) with si-Control and si-4EBP1 transfected K562 cells. This data was developed using the same antibody clone with 60246-1-PBS in a different storage buffer formulation.

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  WB analysis of K-562 using 60246-1-Ig (same clone as 60246-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 60246-1-Ig (4EBP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60246-1-PBS in a different storage buffer formulation.

• 

  WB analysis of K-562 using 60246-1-Ig (same clone as 60246-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 60246-1-Ig (4EBP1 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60246-1-PBS in a different storage buffer formulation.

• 

  WB analysis of RAW 264.7 using 60246-1-Ig (same clone as 60246-1-PBS)

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 60246-1-Ig (4EBP1 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60246-1-PBS in a different storage buffer formulation.

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  WB analysis of NCI-H1299 using 60246-1-Ig (same clone as 60246-1-PBS)

  NCI-H1299 cells were subjected to SDS PAGE followed by western blot with 60246-1-Ig (4EBP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60246-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HCT 116 using 60246-1-Ig (same clone as 60246-1-PBS)

  HCT 116 cells were subjected to SDS PAGE followed by western blot with 60246-1-Ig (4EBP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60246-1-PBS in a different storage buffer formulation.

• 

  WB analysis of C6 using 60246-1-Ig (same clone as 60246-1-PBS)

  C6 cells were subjected to SDS PAGE followed by western blot with 60246-1-Ig (4EBP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60246-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 60246-1-Ig (same clone as 60246-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 60246-1-Ig (4EBP1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60246-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 60246-1-Ig (same clone as 60246-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 60246-1-Ig (4EBP1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60246-1-PBS in a different storage buffer formulation.

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  IHC staining of rat liver using 60246-1-Ig (same clone as 60246-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 60246-1-Ig (4EBP1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60246-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat pancreas using 60246-1-Ig (same clone as 60246-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using 60246-1-Ig (4EBP1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60246-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 60246-1-Ig (same clone as 60246-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60246-1-Ig (4EBP1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60246-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 60246-1-Ig (same clone as 60246-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60246-1-Ig (4EBP1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60246-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 60246-1-Ig (same clone as 60246-1-PBS)

  Immunofluorescent analysis of (-20℃ Ethanol ) fixed HeLa cells using 60246-1-Ig(4EBP1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60246-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60246-1-PBS targets 4EBP1 in WB, IF, IHC, ELISA applications and shows reactivity with human, rat, pig, mouse samples.

• Tested Reactivity: human, rat, pig, mouse
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: 4EBP1 fusion protein Ag18985
• Full Name: eukaryotic translation initiation factor 4E binding protein 1
• Calculated Molecular Weight: 118 aa, 12 kDa
• Observed Molecular Weight: 15-24 kDa
• GenBank Accession Number: BC004459
• Gene Symbol: EIF4EBP1
• Gene ID (NCBI): 1978
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -80°C.

Background Information

Eukaryotic translation initiation factor 4E-binding protein 1(4EBP1), is a member of 4EBPs family, which regulate the translation of a subset of mRNA by competing with eIF4G for binding to eIF4E, thus preventing the assembly of the eIF4F complex. The eIF4F facilitates the recruitment of other translation initiation factors to form the complex and then initiates cap-dependent translation.4EBP1 also mediates the regulation of protein translation by growth factors, hormones and other stimuli that signal through the MAP kinase and mTORC1 pathways. There are three forms of 4EBP1, alpha, beta and gamma.typical pattern seen on Western blots for phosphorylated heat-acid stabled protein 4EBP1 from rat gastrocnemius muscle. Three distinct bands are noted, with the most rapidly migrating band having an apparent molecular mass of ; 20 kDa and the slowest migrating band of; 24 kDa. The 3 bands are designated by convention asalpha, beta and gamma. (PMID: 10913029)