CoraLite® Plus 647-conjugated 14-3-3 Sigma Monoclonal antibody

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  FC experiment of HeLa using CL647-66251

  1X10^6 HeLa cells were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human 14-3-3 Sigma (CL647-66251, Clone:3F10D9) (red), or 0.2 ug APC Mouse IgG2b Isotype Control (APC-65128, Clone: MPC-11) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

14-3-3 Sigma Monoclonal Antibody for FC (Intra)

Cat No. CL647-66251

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

FC (Intra)

Synonyms

14 3 3 protein sigma, 14-3-3 Sigma, HME1, SFN, stratifin, YWHAS

Formulation:  PBS and Azide

PBS and Azide

Conjugate:  CoraLite® Plus 647

Unconjugated CoraLite® Plus 488 CoraLite® Plus 647 CoraLite®555 CoraLite®594

Size/Concentration: 

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Tested Applications

• Positive FC (Intra) detected in: HeLa cells
• Positive FC detected in: HeLa cells

Recommended dilution

• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL647-66251 targets 14-3-3 Sigma in FC (Intra) applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: 14-3-3 Sigma fusion protein Ag12644
• Full Name: stratifin
• Calculated Molecular Weight: 28 kDa
• Observed Molecular Weight: 28-30 kDa
• GenBank Accession Number: BC002995
• Gene Symbol: 14-3-3 Sigma
• Gene ID (NCBI): 2810
• RRID: AB_2935047
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P31947
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

14-3-3 Sigma is a member 14-3-3 family of highly conserved proteins participating in diverse cellular processes including cell cycle progression. It is exclusively present in various epithelial cells, and alternatively named as human epithelial marker (HEM), or stratifin. Alteration of its expression has been linked to cancer.