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14-3-3 Polyclonal antibody
14-3-3 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Cat no : 14503-1-AP
Validation Data Gallery
|Positive WB detected in||HeLa cells, Jurkat cells, rat liver tissue, mouse liver tissue, mouse brain tissue, mouse heart tissue|
|Positive IP detected in||mouse lung tissue|
|Positive IHC detected in||mouse brain tissue, human testis tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Positive FC detected in||HeLa cells|
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
14503-1-AP targets 14-3-3 in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||14-3-3 fusion protein Ag5959|
|Full Name||tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta polypeptide|
|Calculated molecular weight||28 kDa|
|Observed molecular weight||31 kDa|
|GenBank accession number||BC056867|
|Gene ID (NCBI)||10971|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
14-3-3 proteins are the first phosphoserine/phosphothreonine-binding proteins to be discovered. 14-3-3 family members interact with a wide spectrum of proteins and possess diverse functions. Mammals express seven distinct 14-3-3 isoforms (gamma, epsilon, beta, zeta, sigma, theta, tau) that form multiple homo- and hetero- dimmers. 14-3-3 proteins display the highest expression levels in the brain, and have been implicated in several neurodegenerative diseases, including Alzheimer's disease and amyotrophic lateral sclerosis.
|Product Specific Protocols|
|WB protocol for 14-3-3 antibody 14503-1-AP||Download protocol|
|IHC protocol for 14-3-3 antibody 14503-1-AP||Download protocol|
|IF protocol for 14-3-3 antibody 14503-1-AP||Download protocol|
|IP protocol for 14-3-3 antibody 14503-1-AP||Download protocol|
|FC protocol for 14-3-3 antibody 14503-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Aging (Albany NY)
The interaction protein of SORBS2 in myocardial tissue to find out the pathogenic mechanism of LVNC disease.
Int J Mol Sci
APEX2-Mediated Proximity Labeling Resolves the DDIT4-Interacting Proteome.
Chin Med J (Engl)
Regulatory factor X5 promotes hepatocellular carcinoma progression by transactivating tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein theta and suppressing apoptosis.
Mol Biol Cell
Glucocorticoids and Myosin5b loss-of-function induce heightened PKA signaling in addition to membrane traffic defects.
Quantitative proteomic dissection of a native 14-3-3ε interacting protein complex associated with hepatocellular carcinoma.
J Biol Chem
14-3-3 proteins protect AMPK-phosphorylated ten-eleven translocation-2 (TET2) from PP2A-mediated dephosphorylation.