• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

CD122/IL-2RB Recombinant Matched Antibody Pair, PBS Only

Host / Isotype

Capture: Rabbit / IgG

Detection: Rabbit / IgG

Reactivity

Capture: human

Detection: human

Applications

Sandwich ELISA

Range

Sandwich ELISA: 39.1-2500 pg/mL

Cat No : MP01123-3

Print datasheet

Synonyms

Validation Data Gallery

  • Sandwich ELISA standard curve of MP01123-3, Human CD122/IL-2RB Recombinant Matched Antibody Pair - PBS only. 84229-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag3929. 84229-1-PBS was HRP conjugated as the detection antibody. Range: 39.1-2500 pg/mL.

    Sandwich ELISA standard curve of MP01123-3

    Sandwich ELISA standard curve of MP01123-3, Human CD122/IL-2RB Recombinant Matched Antibody Pair - PBS only. 84229-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag3929. 84229-1-PBS was HRP conjugated as the detection antibody. Range: 39.1-2500 pg/mL.

  • Serum of six individual healthy human donors was measured. The CD122/IL-2RB concentration of detected samples was determined to be 92.1 pg/mL with a range of 49.0-189.6 pg/mL.

    Sample test of MP01123-3

    Serum of six individual healthy human donors was measured. The CD122/IL-2RB concentration of detected samples was determined to be 92.1 pg/mL with a range of 49.0-189.6 pg/mL.

  • Human peripheral blood mononuclear cells (PBMC) were cultured unstimulated or stimulated with 10 μg/mL PHA for 3 days. The mean CD122/IL-2RB concentration was not determined in unstimulated PBMC supernatant, 54.6 pg/mL in PHA stimulated PBMC supernatant.

    Sample test of MP01123-3

    Human peripheral blood mononuclear cells (PBMC) were cultured unstimulated or stimulated with 10 μg/mL PHA for 3 days. The mean CD122/IL-2RB concentration was not determined in unstimulated PBMC supernatant, 54.6 pg/mL in PHA stimulated PBMC supernatant.

Tested Applications

Sandwich ELISA

Recommended dilution

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

MP01123-3 targets CD122/IL-2RB in immunoassays as a matched antibody pair. Validated in Sandwich ELISA.

Range: 39.1-2500 pg/mL (Sandwich ELISA);

Capture antibody: IL2RB Recombinant antibody, PBS Only (Capture) 84229-4-PBS (241408G10). 100 μg. Concentration 1 mgl/ml.

Detection antibody: CD122/IL-2RB Recombinant antibody, PBS Only (Detector) 84229-1-PBS (241408A9). 100 μg. Concentration 1 mgl/ml.

Alternative CD122/IL-2RB matched antibody pairs: MP01123-1, MP01123-2, MP50451-1, MP50451-2, MP50451-3

Matched pair protein standard: Ag3929

Unconjugated rabbit recombinant monoclonal antibody pair in PBS only storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

Matched antibody pairs are designed for use in a variety of assays and platforms that require matched antibody pairs. These include:
Sandwich ELISA
Multiplex bead-based arrays
FRET/TR-FRET/HTRF
Simoa® immunoassays (registered trademark of Quanterix, Inc)
AlphaLISA® (registered trademark of PerkinElmer, Inc)
Meso Scale Discovery® (registered trademark of Meso Scale Diagnostics, LLC)
ELLA™ Automated Immunoassay (registered trademark of Bio-Techne Corporation)

Please note we have not tested this product in the above platforms, they are listed for suggestion only as applications for matched antibody pairs. Antibody use should be optimized for each application and assay.

Catalog Number 84229-4-PBS
Tested Reactivity human
Immunogen IL2RB fusion protein Ag3929
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name interleukin 2 receptor, beta
Synonyms IL 2RB, IL 2R subunit beta, IL 2 receptor subunit beta, High affinity IL-2 receptor subunit beta, CD122
GenBank accession numberBC025691
Gene symbol IL2RB
UniprotP14784
Gene ID (NCBI) 3560
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
Catalog Number 84229-1-PBS
Tested Reactivity human
Immunogen CD122/IL-2RB fusion protein Ag3929
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name interleukin 2 receptor, beta
Synonyms IL2RB, IL 2RB, IL 2R subunit beta, IL 2 receptor subunit beta, High affinity IL-2 receptor subunit beta
GenBank accession numberBC025691
Gene symbol IL2RB
UniprotP14784
Gene ID (NCBI) 3560
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.