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MIRO1 Recombinant Matched Antibody Pair, PBS Only

Host / Isotype

Capture: Rabbit / IgG

Detection: Rabbit / IgG

Reactivity

Capture: human

Detection: human

Applications

Sandwich ELISA

Range

Sandwich ELISA: 0.625-40 ng/mL

Cat No : MP01000-4

Print datasheet

Synonyms

Validation Data Gallery

  • Sandwich ELISA standard curve of MP01000-4, Human MIRO1 Recombinant Matched Antibody Pair - PBS only. 84055-7-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag15605. 84055-6-PBS was HRP conjugated as the detection antibody. Range: 0.625-40 ng/mL.

    Sandwich ELISA standard curve of MP01000-4

    Sandwich ELISA standard curve of MP01000-4, Human MIRO1 Recombinant Matched Antibody Pair - PBS only. 84055-7-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag15605. 84055-6-PBS was HRP conjugated as the detection antibody. Range: 0.625-40 ng/mL.

  • The mean MIRO1 concentration was determined to be 17.32 ng/mL in mouse brain cell extract based on a 4.60 mg/mL extract load and 25.35 ng/mL in HeLa cell extract based on a 4.60 mg/mL extract load.

    Sample test of MP01000-4

    The mean MIRO1 concentration was determined to be 17.32 ng/mL in mouse brain cell extract based on a 4.60 mg/mL extract load and 25.35 ng/mL in HeLa cell extract based on a 4.60 mg/mL extract load.

Tested Applications

Sandwich ELISA

Recommended dilution

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

MP01000-4 targets MIRO1 in immunoassays as a matched antibody pair. Validated in Sandwich ELISA.

Range: 0.625-40 ng/mL (Sandwich ELISA);

Capture antibody: RHOT1 Recombinant antibody, PBS Only (Capture) 84055-7-PBS (241157A9). 100 μg. Concentration 1 mgl/ml.

Detection antibody: RHOT1 Recombinant antibody, PBS Only (Detector) 84055-6-PBS (241157F10). 100 μg. Concentration 1 mgl/ml.

Alternative MIRO1 matched antibody pairs: MP01000-1, MP01000-2, MP01000-3

Matched pair protein standard: Ag15605

Unconjugated rabbit recombinant monoclonal antibody pair in PBS only storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

Matched antibody pairs are designed for use in a variety of assays and platforms that require matched antibody pairs. These include:
Sandwich ELISA
Multiplex bead-based arrays
FRET/TR-FRET/HTRF
Simoa® immunoassays (registered trademark of Quanterix, Inc)
AlphaLISA® (registered trademark of PerkinElmer, Inc)
Meso Scale Discovery® (registered trademark of Meso Scale Diagnostics, LLC)
ELLA™ Automated Immunoassay (registered trademark of Bio-Techne Corporation)

Please note we have not tested this product in the above platforms, they are listed for suggestion only as applications for matched antibody pairs. Antibody use should be optimized for each application and assay.

Catalog Number 84055-7-PBS
Tested Reactivity human
Immunogen RHOT1 fusion protein Ag15605
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name ras homolog gene family, member T1
Synonyms Rac-GTP-binding protein-like protein, MIRO 1, hMiro 1, EC:3.6.5.-, ARHT1
GenBank accession numberBC060781
Gene symbol MIRO1
UniprotQ8IXI2
Gene ID (NCBI) 55288
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
Catalog Number 84055-6-PBS
Tested Reactivity human
Immunogen RHOT1 fusion protein Ag15605
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name ras homolog gene family, member T1
Synonyms Rac-GTP-binding protein-like protein, MIRO 1, hMiro 1, EC:3.6.5.-, ARHT1
GenBank accession numberBC060781
Gene symbol MIRO1
UniprotQ8IXI2
Gene ID (NCBI) 55288
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.