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EPAS1 Recombinant Matched Antibody Pair, PBS Only

Host / Isotype

Capture: Rabbit / IgG

Detection: Rabbit / IgG

Reactivity

Capture: human

Detection: human

Applications

Sandwich ELISA

Range

Sandwich ELISA: 62.5-4000 pg/mL

Cat No : MP00775-4

Print datasheet

Synonyms

Validation Data Gallery

  • Sandwich ELISA standard curve of MP00775-4, Human EPAS1 Recombinant Matched Antibody Pair - PBS only. 83790-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag15199. 83790-5-PBS was HRP conjugated as the detection antibody. Range: 62.5-4000 pg/mL

    Sandwich ELISA standard curve of MP00775-4

    Sandwich ELISA standard curve of MP00775-4, Human EPAS1 Recombinant Matched Antibody Pair - PBS only. 83790-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag15199. 83790-5-PBS was HRP conjugated as the detection antibody. Range: 62.5-4000 pg/mL

  • A549 and Hela cell lysates were measured. The human EPAS1 concentration of detected samples was determined to be 7,062.3 pg/mL (based on a 1.00 mg/mL extract load) in A549 cell lysate and 6,988.6 pg/mL (based on a 1.50 mg/mL extract load) in Hela cell lysate.

    Sample test of MP00775-4

    A549 and Hela cell lysates were measured. The human EPAS1 concentration of detected samples was determined to be 7,062.3 pg/mL (based on a 1.00 mg/mL extract load) in A549 cell lysate and 6,988.6 pg/mL (based on a 1.50 mg/mL extract load) in Hela cell lysate.

Tested Applications

Sandwich ELISA

Recommended dilution

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

MP00775-4 targets EPAS1 in immunoassays as a matched antibody pair. Validated in Sandwich ELISA.

Range: 62.5-4000 pg/mL (Sandwich ELISA);

Capture antibody: EPAS1 Recombinant antibody, PBS Only (Capture) 83790-4-PBS (240736H7). 100 μg. Concentration 1 mgl/ml.

Detection antibody: EPAS1 Recombinant antibody, PBS Only (Detector) 83790-5-PBS (240736C9). 100 μg. Concentration 1 mgl/ml.

Alternative EPAS1 matched antibody pairs: MP00775-1, MP00775-2, MP00775-3

Matched pair protein standard: Ag15199

Unconjugated rabbit recombinant monoclonal antibody pair in PBS only storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

Matched antibody pairs are designed for use in a variety of assays and platforms that require matched antibody pairs. These include:
Sandwich ELISA
Multiplex bead-based arrays
FRET/TR-FRET/HTRF
Simoa® immunoassays (registered trademark of Quanterix, Inc)
AlphaLISA® (registered trademark of PerkinElmer, Inc)
Meso Scale Discovery® (registered trademark of Meso Scale Diagnostics, LLC)
ELLA™ Automated Immunoassay (registered trademark of Bio-Techne Corporation)

Please note we have not tested this product in the above platforms, they are listed for suggestion only as applications for matched antibody pairs. Antibody use should be optimized for each application and assay.

Catalog Number 83790-4-PBS
Tested Reactivity human
Immunogen EPAS1 fusion protein Ag15199
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name endothelial PAS domain protein 1
Synonyms Endothelial PAS domain-containing protein 1, ECYT4, Class E basic helix-loop-helix protein 73, bHLHe73, Basic-helix-loop-helix-PAS protein MOP2
GenBank accession numberBC051338
Gene symbol HIF2α
UniprotQ99814
Gene ID (NCBI) 2034
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
Catalog Number 83790-5-PBS
Tested Reactivity human
Immunogen EPAS1 fusion protein Ag15199
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name endothelial PAS domain protein 1
Synonyms Endothelial PAS domain-containing protein 1, ECYT4, Class E basic helix-loop-helix protein 73, bHLHe73, Basic-helix-loop-helix-PAS protein MOP2
GenBank accession numberBC051338
Gene symbol HIF2α
UniprotQ99814
Gene ID (NCBI) 2034
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.