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CTLA-4/CD152 Recombinant Matched Antibody Pair, PBS Only

Host / Isotype

Capture: Rabbit / IgG

Detection: Rabbit / IgG

Reactivity

Capture: human

Detection: human

Applications

Sandwich ELISA

Range

Sandwich ELISA: 23.4-1500 pg/mL

Cat No : MP00736-4

Print datasheet

Synonyms

Validation Data Gallery

  • Sandwich ELISA standard curve of MP00736-4, Human CTLA4/CD152 Recombinant Matched Antibody Pair - PBS only. 83778-6-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0972. 83778-3-PBS was HRP conjugated as the detection antibody. Range: 23.4-1500 pg/mL.

    Sandwich ELISA standard curve of MP00736-4

    Sandwich ELISA standard curve of MP00736-4, Human CTLA4/CD152 Recombinant Matched Antibody Pair - PBS only. 83778-6-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0972. 83778-3-PBS was HRP conjugated as the detection antibody. Range: 23.4-1500 pg/mL.

  • Serum of eight individual healthy human donors was measured. The human CTLA4/CD152 concentration of detected samples was determined to be 39.8 pg/mL with a range of ND-151.6 pg/mL.

    Sample test of MP00736-4

    Serum of eight individual healthy human donors was measured. The human CTLA4/CD152 concentration of detected samples was determined to be 39.8 pg/mL with a range of ND-151.6 pg/mL.

  • The mean CTLA4/CD152 concentration was determined to be 3,592.9 pg/mL in A549 cell extract based on a 2.2 mg/mL extract load.

    Sample test of MP00736-4

    The mean CTLA4/CD152 concentration was determined to be 3,592.9 pg/mL in A549 cell extract based on a 2.2 mg/mL extract load.

Tested Applications

Sandwich ELISA

Recommended dilution

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

MP00736-4 targets CTLA-4/CD152 in immunoassays as a matched antibody pair. Validated in Sandwich ELISA.

Range: 23.4-1500 pg/mL (Sandwich ELISA);

Capture antibody: CTLA-4/CD152 Recombinant antibody, PBS Only (Capture) 83778-6-PBS (240793E6). 100 μg. Concentration 1 mgl/ml.

Detection antibody: CTLA-4/CD152 Recombinant antibody, PBS Only (Detector) 83778-3-PBS (240793F3). 100 μg. Concentration 1 mgl/ml.

Alternative CTLA-4/CD152 matched antibody pairs: MP00736-1, MP00736-2, MP00736-3, MP00907-1, MP00907-2, MP00907-3, MP00907-4, MP50106-1, MP50106-2

Matched pair protein standard: Eg0972

Unconjugated rabbit recombinant monoclonal antibody pair in PBS only storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

Matched antibody pairs are designed for use in a variety of assays and platforms that require matched antibody pairs. These include:
Sandwich ELISA
Multiplex bead-based arrays
FRET/TR-FRET/HTRF
Simoa® immunoassays (registered trademark of Quanterix, Inc)
AlphaLISA® (registered trademark of PerkinElmer, Inc)
Meso Scale Discovery® (registered trademark of Meso Scale Diagnostics, LLC)
ELLA™ Automated Immunoassay (registered trademark of Bio-Techne Corporation)

Please note we have not tested this product in the above platforms, they are listed for suggestion only as applications for matched antibody pairs. Antibody use should be optimized for each application and assay.

Catalog Number 83778-6-PBS
Tested Reactivity human
Immunogen Fusion Protein
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name cytotoxic T-lymphocyte-associated protein 4
Synonyms CTLA4, Cytotoxic T-lymphocyte protein 4, CTLA 4, CELIAC3, CD152
GenBank accession numberBC070162
Gene symbol CTLA4
UniprotP16410
Gene ID (NCBI) 1493
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
Catalog Number 83778-3-PBS
Tested Reactivity human
Immunogen Fusion Protein
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name cytotoxic T-lymphocyte-associated protein 4
Synonyms CTLA4, Cytotoxic T-lymphocyte protein 4, CTLA 4, CELIAC3, CD152
GenBank accession numberBC070162
Gene symbol CTLA4
UniprotP16410
Gene ID (NCBI) 1493
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.