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ACOX1 Recombinant Matched Antibody Pair, PBS Only

Host / Isotype

Capture: Rabbit / IgG

Detection: Rabbit / IgG

Reactivity

Capture: human

Detection: human, mouse, rat

Applications

Cytometric bead array, Sandwich ELISA

Range

Cytometric Bead Array: 0.78-50 ng/mL

Sandwich ELISA: 0.156-10 ng/mL

Cat No : MP00709-3

Print datasheet

Synonyms

Validation Data Gallery

  • Sandwich ELISA standard curve of MP00709-3, Human AOX Recombinant Matched Antibody Pair - PBS only. 83731-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag1405. 83731-2-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL

    Sandwich ELISA standard curve of MP00709-3

    Sandwich ELISA standard curve of MP00709-3, Human AOX Recombinant Matched Antibody Pair - PBS only. 83731-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag1405. 83731-2-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL

  • K-562 and U-251 cell lysates were measured. The human AOX concentration of detected samples was determined to be 158.79 ng/mL (based on a 2.1 mg/mL extract load) in K-562 cell lyaste and 345.46 ng/mL (based on a 1.6 mg/mL extract load) in U-251 cell lysate.

    Sample test of MP00709-3

    K-562 and U-251 cell lysates were measured. The human AOX concentration of detected samples was determined to be 158.79 ng/mL (based on a 2.1 mg/mL extract load) in K-562 cell lyaste and 345.46 ng/mL (based on a 1.6 mg/mL extract load) in U-251 cell lysate.

  • Cytometric bead array standard curve of MP00709-3, AOX Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83731-1-PBS. Detection antibody: 83731-2-PBS. Standard: Ag1405. Range: 0.78-50 ng/mL.

    Cytometric bead array standard curve of MP00709-3

    Cytometric bead array standard curve of MP00709-3, AOX Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83731-1-PBS. Detection antibody: 83731-2-PBS. Standard: Ag1405. Range: 0.78-50 ng/mL.

Tested Applications

Cytometric bead array, Sandwich ELISA

Recommended dilution

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

MP00709-3 targets ACOX1 in immunoassays as a matched antibody pair. Validated in Cytometric bead array, Sandwich ELISA.

Range: 0.78-50 ng/mL (Cytometric Bead Array); 0.156-10 ng/mL (Sandwich ELISA);

Capture antibody: ACOX1 Recombinant antibody, PBS Only (Capture) 83731-1-PBS (240754D3). 100 μg. Concentration 1 mgl/ml.

Detection antibody: ACOX1 Recombinant antibody, PBS Only (Detector) 83731-2-PBS (240754E3). 100 μg. Concentration 1 mgl/ml.

Alternative ACOX1 matched antibody pairs: MP00709-2

Matched pair protein standard: Ag1405

Unconjugated rabbit recombinant monoclonal antibody pair in PBS only storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

Matched antibody pairs are designed for use in a variety of assays and platforms that require matched antibody pairs. These include:
Sandwich ELISA
Multiplex bead-based arrays
FRET/TR-FRET/HTRF
Simoa® immunoassays (registered trademark of Quanterix, Inc)
AlphaLISA® (registered trademark of PerkinElmer, Inc)
Meso Scale Discovery® (registered trademark of Meso Scale Diagnostics, LLC)
ELLA™ Automated Immunoassay (registered trademark of Bio-Techne Corporation)

Please note we have not tested this product in the above platforms, they are listed for suggestion only as applications for matched antibody pairs. Antibody use should be optimized for each application and assay.

Catalog Number 83731-1-PBS
Tested Reactivity human
Immunogen ACOX1 fusion protein Ag1405
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name acyl-Coenzyme A oxidase 1, palmitoyl
Synonyms Palmitoyl-CoA oxidase, EC:1.3.3.6, AOX, ACOX 1, ACOX
GenBank accession numberBC010425
Gene symbol ACOX1
UniprotQ15067
Gene ID (NCBI) 51
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
Catalog Number 83731-2-PBS
Tested Reactivity human, mouse, rat
Immunogen ACOX1 fusion protein Ag1405
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name acyl-Coenzyme A oxidase 1, palmitoyl
Synonyms Palmitoyl-CoA oxidase, EC:1.3.3.6, AOX, ACOX 1, ACOX
GenBank accession numberBC010425
Gene symbol ACOX1
UniprotQ15067
Gene ID (NCBI) 51
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.