ACOX1 Recombinant antibody, PBS Only (Detector)

ACOX1 Uni-rAb^TM Recombinant Antibody for WB, IHC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

240754E3

Cat no : 83731-2-PBS

Synonyms

Palmitoyl-CoA oxidase, EC:1.3.3.6, AOX, ACOX 1, ACOX

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Validation Data Gallery

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  WB analysis using 83731-2-RR (same clone as 83731-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83731-2-RR (ACOX1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83731-2-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP00709-3

  Sandwich ELISA standard curve of MP00709-3, Human AOX Recombinant Matched Antibody Pair - PBS only. 83731-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag1405. 83731-2-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL

• 

  Sample test of MP00709-3

  K-562 and U-251 cell lysates were measured. The human AOX concentration of detected samples was determined to be 158.79 ng/mL (based on a 2.1 mg/mL extract load) in K-562 cell lyaste and 345.46 ng/mL (based on a 1.6 mg/mL extract load) in U-251 cell lysate.

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  IHC staining of human ovarian cancer using 83731-2-RR (same clone as 83731-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 83731-2-RR (ACOX1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83731-2-PBS in a different storage buffer formulation.

• 

  FC experiment of A431 using 83731-2-RR (same clone as 83731-2-PBS)

  1x10^6 A431 cells were intracellularly stained with 0.25 ug ACOX1 Recombinant antibody (83731-2-RR, Clone:240754E3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83731-2-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00709-2

  Cytometric bead array standard curve of MP00709-2, AOX Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83731-3-PBS. Detection antibody: 83731-2-PBS. Standard: Ag1405. Range: 1.56-100 ng/mL.

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  Cytometric bead array standard curve of MP00709-3

  Cytometric bead array standard curve of MP00709-3, AOX Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83731-1-PBS. Detection antibody: 83731-2-PBS. Standard: Ag1405. Range: 0.78-50 ng/mL.

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  Affinity and Kinetic Characterization of 83731-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83731-2-RR against Human ACOX1 were performed. The affinity constant is below 1 pM.

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Product Information

83731-2-PBS targets ACOX1 as part of a matched antibody pair:

MP00709-2: 83731-3-PBS capture and 83731-2-PBS detection (validated in Cytometric bead array)

MP00709-3: 83731-1-PBS capture and 83731-2-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: ACOX1 fusion protein Ag1405
• Full Name: acyl-Coenzyme A oxidase 1, palmitoyl
• Calculated Molecular Weight: 74 kDa
• Observed Molecular Weight: 70-74 kDa, 22 kDa
• GenBank Accession Number: BC010425
• Gene Symbol: ACOX1
• Gene ID (NCBI): 51
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

ACOX1(Peroxisomal acyl-coenzyme A oxidase 1) is the first and rate-limiting enzyme in the peroxisomal fatty acid beta-oxidation pathway and catalyzes the desaturation of acyl-CoAs to 2-trans-enoyl-CoAs. It belongs to the acyl-CoA oxidase family and also named as ACOX, AOX, SCOX. It has 3 isoforms produced by alternative splicing with the molecular mass of 70-74 kDa and can be cleaved post-translationally into a 50-kDa and a 22-kDa subunit between Val468 and Ala469 (PMID: 10672038). Defects in ACOX1 are the cause of adrenoleukodystrophy pseudoneonatal (Pseudo-NALD).