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PSMD1 Recombinant Matched Antibody Pair, PBS Only

Host / Isotype

Capture: Rabbit / IgG

Detection: Rabbit / IgG

Reactivity

Capture: human, mouse, rat

Detection: human

Applications

Sandwich ELISA

Range

Sandwich ELISA: 78.1-5000 pg/mL

Cat no : MP00519-3

Synonyms

Validation Data Gallery

  • Sandwich ELISA standard curve of MP00519-3, Human PSMD1 Recombinant Matched Antibody Pair - PBS only. 83521-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag12009. 83521-5-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL

    Sandwich ELISA standard curve of MP00519-3

    Sandwich ELISA standard curve of MP00519-3, Human PSMD1 Recombinant Matched Antibody Pair - PBS only. 83521-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag12009. 83521-5-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL

  • Mouse stomach tissue lysate and Jurkat cell lysate were measured. The human PSMD1 concentration of detected samples was determined to be 11,510.8 pg/mL in mouse stomach lysate (based on a 4.0 mg/mL extract load) and 17,515.2 pg/mL in Jurkat cell lysate (based on a 3.4 mg/mL extract load).

    Sample test of MP00519-3

    Mouse stomach tissue lysate and Jurkat cell lysate were measured. The human PSMD1 concentration of detected samples was determined to be 11,510.8 pg/mL in mouse stomach lysate (based on a 4.0 mg/mL extract load) and 17,515.2 pg/mL in Jurkat cell lysate (based on a 3.4 mg/mL extract load).

Tested Applications

Sandwich ELISA

Recommended dilution

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

MP00519-3 targets PSMD1 in immunoassays as a matched antibody pair. Validated in Sandwich ELISA.

Range: 78.1-5000 pg/mL (Sandwich ELISA);

Capture antibody: PSMD1 Recombinant antibody, PBS Only (Capture/Detector) 83521-2-PBS (240458B3). 100 μg. Concentration 1 mg/ml.

Detection antibody: PSMD1 Recombinant antibody, PBS Only (Detector) 83521-5-PBS (240458C4). 100 μg. Concentration 1 mg/ml.

Alternative PSMD1 matched antibody pairs: MP00519-1, MP00519-2

Matched pair protein standard: Ag12009

Unconjugated rabbit recombinant monoclonal antibody pair in PBS only storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

Matched antibody pairs are designed for use in a variety of assays and platforms that require matched antibody pairs. These include:
Sandwich ELISA
Multiplex bead-based arrays
FRET/TR-FRET/HTRF
Simoa® immunoassays (registered trademark of Quanterix, Inc)
AlphaLISA® (registered trademark of PerkinElmer, Inc)
Meso Scale Discovery® (registered trademark of Meso Scale Diagnostics, LLC)
ELLA™ Automated Immunoassay (registered trademark of Bio-Techne Corporation)

Please note we have not tested this product in the above platforms, they are listed for suggestion only as applications for matched antibody pairs. Antibody use should be optimized for each application and assay.

Catalog Number 83521-2-PBS
Tested Reactivity human, mouse, rat
Immunogen PSMD1 fusion protein Ag12009
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name proteasome (prosome, macropain) 26S subunit, non-ATPase, 1
Synonyms PSMD 1, 26S proteasome non-ATPase regulatory subunit 1, 240458B3
GenBank accession numberBC094720
Gene symbol PSMD1
UniprotQ99460
Gene ID (NCBI) 5707
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
Catalog Number 83521-5-PBS
Tested Reactivity human
Immunogen PSMD1 fusion protein Ag12009
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name proteasome (prosome, macropain) 26S subunit, non-ATPase, 1
Synonyms PSMD 1, 26S proteasome non-ATPase regulatory subunit 1, 240458C4
GenBank accession numberBC094720
Gene symbol PSMD1
UniprotQ99460
Gene ID (NCBI) 5707
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.