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  • HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human Thrombin (Coagulation Factor II) protein

Activity(Primary)

1000-5000 units/mg

Species Reactivity

human,mouse

Purity

> 95%

Cat no : HZ-3010

Synonyms

Coagulation factor II, F2, Prothrombin, PT, Thrombin

Validation Data Gallery

  • Purity of recombinant human thrombin was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

    Purity of recombinant human thrombin was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

  • The activity of GMP thrombin (HZ-3010-GMP) was measured by the rate of free chromophore formation from Chromozym TH thrombin substrate monitored by absorbance at 405 nm. The enzymatic reaction was carried out in 50mM Tris-HCl pH 8.3 buffer containing 0.1% BSA and 227 mM NaCl at 25°C. Activity ranges from 1000-5000 units/mg.

    The activity of GMP thrombin (HZ-3010-GMP) was measured by the rate of free chromophore formation from Chromozym TH thrombin substrate monitored by absorbance at 405 nm. The enzymatic reaction was carried out in 50mM Tris-HCl pH 8.3 buffer containing 0.1% BSA and 227 mM NaCl at 25°C. Activity ranges from 1000-5000 units/mg.

Technical Specifications

GeneID 2147
Species Human
Expression HEK293
Activity(Primary) 1000-5000 units/mg
Purity > 95%
Endotoxin<1 EU/µg
Accession NumberP00734
Molecular Mass 34 kDa reduced, 36 kDa non-reduced, monomer, glycosylated
Formulation 20 mM MES pH 6.0 + 500 mM Choline Chloride, See Certificate of Analysis for details
Species Reactivity human,mouse

Stability and Reconstitution

Stability and StorageThis protein is stable for 1 year from the date of receipt if stored between (-20°C) and (-80°C). Repeated freeze thaw cycles should be avoided.
Reconstitution Not Applicable. Product provided in liquid format.

Background

Coagulation factors are a group of related proteins that are essential for normal blood clotting (haemostasis). After an injury, clots protect the body by sealing off damaged blood vessels and preventing further blood loss. Prothrombin circulates in the bloodstream in an inactive form until an injury occurs. In response to that, prothrombin is converted to its active form, thrombin. Thrombin next converts a protein called fibrinogen into fibrin. Thrombin is also important for cell growth and division, tissue repair, and new blood vessels formation - angiogenesis (PMID: 11154146; 16549895; 15892853; 12421139).

Synonyms

Coagulation factor II, F2, Prothrombin, PT, Thrombin

Publications

SpeciesTitle
mouse

Clin Ther

A review of three stand-alone topical thrombins for surgical hemostasis.

Authors - Christine MCheng
View Article