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  • HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human EGF protein

Activity(Primary)

0.1-0.6 ng/mL

Specific Activity

minimally 1.00x106 IU/mg

Species Reactivity

human,mouse,rat

Purity

> 95%

Cat no : HZ-1326

Synonyms

Urogastrone

Validation Data Gallery

  • Purity of recombinant human EGF was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

    Purity of recombinant human EGF was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

  • Recombinant human EGF (HZ-1326) induces dose-dependent proliferation of the 4MBr-5 (monkey epithelial) cell line. Cell number was quantitatively assessed by PrestoBlue® cell viability reagent. 4MBr-5 cells were treated with increasing concentrations of recombinant EGF for 120 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 0.1-0.6 ng/mL.

    Recombinant human EGF (HZ-1326) induces dose-dependent proliferation of the 4MBr-5 (monkey epithelial) cell line. Cell number was quantitatively assessed by PrestoBlue® cell viability reagent. 4MBr-5 cells were treated with increasing concentrations of recombinant EGF for 120 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 0.1-0.6 ng/mL.

Technical Specifications

GeneID 1950
Species Human
Expression HEK293
Activity(Primary) 0.1-0.6 ng/mL
Specific Activityminimally 1.00x106 IU/mg
Purity > 95%
Endotoxin<1.0 EU/µg
Accession NumberP01133
Molecular Mass 6 kDa reduced, 10 kDa non-reduced, monomer, glycosylated
Formulation 1x PBS, See Certificate of Analysis for details
Species Reactivity human,mouse,rat

Stability and Reconstitution

Stability and StorageProduct FormTemperature ConditionsStorage Time
(From Date of Receipt)
Lyophilized-20°C to -80°CUntil Expiry Date
LyophilizedRoom Temperature2 weeks
Reconstituted as per CofA-20°C to -80°C6 months
Reconstituted as per CofA4°C1 week
Avoid repeated freeze-thaw cycles.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 1x PBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA). Gently swirl or tap vial to mix.

Background

EGF (epidermal growth factor) is a 6 kDA, single chain protein that serves a mitogenic factor for numerous cell types, particularly epithelial cells and fibroblasts. It is predominantly produced and secreted by the kidneys in humans. EGF activates cells through binding with its corresponding receptor (EGFR). Receptor binding activates several downstream pathways leading to cell proliferation, differentiation, and apoptosis. EGF plays important roles in embryonic development, tissue regeneration, transport through ion channels, and wound healing. Dysregulated EGF signaling has been linked with renal disease progression, and EGF levels could potentially be used as biomarker to monitor disease-related cell damage (PMID: 15110798, 24513230, 27868030).

Synonyms

Urogastrone

Publications

SpeciesTitle
mouse

Sci Adv

Varying mechanical forces drive sensory epithelium formation

Authors - Mingyu Xia
View Article
mouse

Nat Commun

Pharmacological blockade of TEAD–YAP reveals its therapeutic limitation in cancer cells

Authors - Yang Sun
View Article
mouse

Am J Physiol Renal Physiol

A conditionally immortalized Gli1-positive kidney mesenchymal cell line models myofibroblast transition

Authors - Eoghainín Ó hAinmhire
View Article
human

Cancer Med

Integrated analysis based on vesicle trafficking-related genes identifying CNIH4 as a novel therapeutic target for glioma

Authors - Zhen Fang
View Article
human

Med Sci Monit

Unveiling the Differences in Biological Properties of Dental Pulp Stem Cells from Normal and Inflamed Pulp: A Comprehensive Comparative Study.

Authors - Shao-Chen Nie
View Article
mouse

Front Pharmacol

Bu-Gu-Sheng-Sui decoction promotes osteogenesis via activating the ERK/Smad signaling pathways

Authors - Ning Liu
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

K. (Verified Customer) (10-26-2023)

Used it in 100-500ng concentration for 10-30 minutes to check EGFR activation and always worked well.

  • Applications: Western Blot,Immunofluorescence,Cell culture
  • Primary Antibody Dilution: 200ng
  • Cell Tissue Type: Human Liver cells