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  • HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human VEGF121 protein

Activity(Primary)

Typically ≤ 15 ng/mL

Species Reactivity

human,pig,mouse

Purity

>95%

Cat no : HZ-1204

Synonyms

L VEGFA, MVCD1, Vascular permeability factor, VEGF, VEGF A, VEGF121, VEGFA, VPF

Validation Data Gallery

  • Purity of recombinant human VEGF121 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

    Purity of recombinant human VEGF121 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

  • Recombinant human VEGF121 (HZ-1204) induces dose-dependent proliferation of the HUVEC (human umbilical vein endothelial) cell line. Cell number was quantitatively assessed by PrestoBlue® cell viability reagent. HUVEC cells were treated with increasing concentrations of recombinant VEGF121 for 96 hours. The EC50 was determined using a 4-parameter non-linear regression model. Activity determination was conducted in triplicate on a validated bioassay. The EC50 range is less than 15 ng/mL.

    Recombinant human VEGF121 (HZ-1204) induces dose-dependent proliferation of the HUVEC (human umbilical vein endothelial) cell line. Cell number was quantitatively assessed by PrestoBlue® cell viability reagent. HUVEC cells were treated with increasing concentrations of recombinant VEGF121 for 96 hours. The EC50 was determined using a 4-parameter non-linear regression model. Activity determination was conducted in triplicate on a validated bioassay. The EC50 range is less than 15 ng/mL.

Technical Specifications

GeneID 7422
Species Human
Expression HEK293
Activity(Primary) Typically ≤ 15 ng/mL
Purity >95%
Endotoxin<1 EU/µg
Accession NumberP15692
Molecular Mass 17 and 21 kDa reduced, 28 to 40 kDa non-reduced, homodimer and homotrimer, glycosylated
Formulation 1x PBS, See Certificate of Analysis for details
Species Reactivity human,pig,mouse

Stability and Reconstitution

Stability and StorageProduct FormTemperature ConditionsStorage Time
(From Date of Receipt)
Lyophilized-20°C to -80°CUntil Expiry Date
LyophilizedRoom Temperature2 weeks
Reconstituted as per CofA-20°C to -80°C6 months
Reconstituted as per CofA4°C1 week
Avoid repeated freeze-thaw cycles.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 1xPBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA). Gently swirl or tap vial to mix.

Background

This gene is a member of the PDGF/VEGF growth factor family. It encodes a heparin-binding protein, which exists as a disulphide-linked homodimer. This growth factor induces proliferation and migration of vascular endothelial cells and is essential for physiological and pathological angiogenesis. Misregulations of this gene in mice result in abnormal embryonic blood vessel formation (PMID:10838264; 11329058).

Synonyms

L VEGFA, MVCD1, Vascular permeability factor, VEGF, VEGF A, VEGF121, VEGFA, VPF

Publications

SpeciesTitle
mouse,human

Circ Res

Esm1 modulates endothelial tip cell behavior and vascular permeability by enhancing VEGF bioavailability.

Authors - Susana FRocha
View Article
human

J Clin Invest

Sema3E-PlexinD1 signaling selectively suppresses disoriented angiogenesis in ischemic retinopathy in mice.

Authors - YokoFukushima
View Article

Nat Commun

DutaFabs are engineered therapeutic Fab fragments that can bind two targets simultaneously.

Authors - Roland Beckmann
View Article
human

Elife

Generation of an expandable intermediate mesoderm restricted progenitor cell line from human pluripotent stem cells.

Authors - NathanKumar
View Article
human

Oncogene

Progranulin and granulin-like protein as novel VEGF-independent angiogenic factors derived from human mesothelioma cells.

Authors - REguchi
View Article
human

Protein Cell

CRISPR/Cas9-mediated gene knockout reveals a guardian role of NF-κB/RelA in maintaining the homeostasis of human vascular cells.

Authors - Ping Wang
View Article