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  • HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human FLT3 Ligand protein

Activity(Primary)

0.4-3.0 ng/mL

Specific Activity

minimally 7.00 x 105 IU/mg

Species Reactivity

human,mouse

Purity

>95%

Cat no : HZ-1151

Synonyms

FL, Flt3 ligand, Flt3L, FLT3LG, SL cytokine

Validation Data Gallery

  • Purity of recombinant human FLT3 Ligand was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

    Purity of recombinant human FLT3 Ligand was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

  • Recombinant human FLT3 (HZ-1151) Ligand stimulates dose-dependent proliferation of the human acute myeloid leukemia cell line (OCI-AML5). Viable cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. OCI-AML5 cells were treated with increasing concentrations of recombinant human FLT3 Ligand for 72 hours. The EC50 was determined using a 4- parameter non-linear regression model. The EC50 values range is 0.4-3 ng/mL.

    Recombinant human FLT3 (HZ-1151) Ligand stimulates dose-dependent proliferation of the human acute myeloid leukemia cell line (OCI-AML5). Viable cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. OCI-AML5 cells were treated with increasing concentrations of recombinant human FLT3 Ligand for 72 hours. The EC50 was determined using a 4- parameter non-linear regression model. The EC50 values range is 0.4-3 ng/mL.

Technical Specifications

GeneID 2323
Species Human
Expression HEK293
Activity(Primary) 0.4-3.0 ng/mL
Specific Activityminimally 7.00 x 105 IU/mg
Purity >95%
Endotoxin<1 EU/µg
Accession NumberP49771
Molecular Mass 27 to 34 kDa reduced, 23 to 30 kDa non-reduced, monomer, glycosylated
Formulation 1x PBS, See Certificate of Analysis for details
Species Reactivity human,mouse

Stability and Reconstitution

Stability and StorageProduct FormTemperature ConditionsStorage Time
(From Date of Receipt)
Lyophilized-20°C to -80°CUntil Expiry Date
LyophilizedRoom Temperature2 weeks
Reconstituted as per CofA-20°C to -80°C6 months
Reconstituted as per CofA4°C1 week
Avoid repeated freeze-thaw cycles.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile water containing 0.1% endotoxin-free recombinant human serum albumin (HSA). Gently swirl or tap vial to mix.

Background

FMS-related tyrosine kinase 3 ligand (FLT3 Ligand) is a growth factor that regulates proliferation of early hematopoietic cells. FLT3 Ligand binds to cells expressing the tyrosine kinase receptor FLT3. FLT3 Ligand by itself does not stimulate proliferation of early hematopoietic cells, but synergizes with other CSFs and interleukins to induce growth and differentiation (PMID: 8590775; 7544638; 12176867).

Synonyms

FL, Flt3 ligand, Flt3L, FLT3LG, SL cytokine

Publications

SpeciesTitle
human

Cell Res

Three-dimensional bioprinted glioblastoma microenvironments model cellular dependencies and immune interactions.

Authors - Min Tang
View Article
human

Blood

LIN28B-mediated expression of fetal hemoglobin and production of fetal-like erythrocytes from adult human erythroblasts ex vivo.

Authors - Y TerryLee
View Article
human

J Exp Med

Co-inhibition of NF-κB and JNK is synergistic in TNF-expressing human AML.

Authors - AndrewVolk
View Article

Dev Cell

Defining the identity and the niches of epithelial stem cells with highly pleiotropic multilineage potency in the human thymus

Authors - Roberta Ragazzini
View Article
human

Stem Cells

Brief report: efficient generation of hematopoietic precursors and progenitors from human pluripotent stem cell lines.

Authors - Niels-BjarneWoods
View Article
human

ACS Biomater Sci Eng

Bioprinting of Human Cord Blood-Derived CD34+ Cells and Exploration of the Multilineage Differentiation Ability in Vitro.

Authors - Lidan Chen
View Article