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  • HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human FLT3 Ligand protein- GMP grade

EC50

0.4-3.0 ng/mL

Specific Activity

minimally 7.00 x 105 IU/mg, typically 7.24 x 105 IU/mg

Species Reactivity

human,mouse

Purity

>95%

ISO 13485 certified HumanKine® human cell-expressed cytokines and growth factors

Cat no : HZ-1151-GMP

Synonyms

FL, Flt3 ligand, Flt3L, FLT3LG, SL cytokine

Validation Data Gallery

  • Purity of GMP-grade recombinant human FLT3 Ligand was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

    Purity of GMP-grade recombinant human FLT3 Ligand was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

  • GMP-grade recombinant human FLT3 Ligand (HZ-1151-GMP) stimulates dose-dependent proliferation of the human acute myeloid leukemia cell line (OCI-AML5). Viable cell number was quantitiatively assessed by PrestoBlue Cell Viability Reagent. OCI-AML5 cells were treated with increasing concentrations of recombinant human FLT3 Ligand for 72 hours.The EC50 was determined using a 4- parameter non-linear regression model. Activity determination was conducted in triplicate on a validated bioassay. The EC50 value ranges from 0.4-3.0 ng/mL.

    GMP-grade recombinant human FLT3 Ligand (HZ-1151-GMP) stimulates dose-dependent proliferation of the human acute myeloid leukemia cell line (OCI-AML5). Viable cell number was quantitiatively assessed by PrestoBlue Cell Viability Reagent. OCI-AML5 cells were treated with increasing concentrations of recombinant human FLT3 Ligand for 72 hours.The EC50 was determined using a 4- parameter non-linear regression model. Activity determination was conducted in triplicate on a validated bioassay. The EC50 value ranges from 0.4-3.0 ng/mL.

Technical Specifications

GeneID 2323
Species Human
Expression HEK293
EC50 0.4-3.0 ng/mL
Specific Activityminimally 7.00 x 105 IU/mg, typically 7.24 x 105 IU/mg
Purity >95%
Endotoxin<0.1 EU/µg
Accession NumberP49771
Molecular Mass 27 to 34 kDa reduced, 23 to 30 kDa non-reduced, monomer, glycosylated
Formulation 1x PBS, See Certificate of Analysis for details
Species Reactivity human,mouse

Stability and Reconstitution

Stability and StorageProduct FormTemperature ConditionsStorage Time
(From Date of Receipt)
Lyophilized-20°C to -80°CUntil Expiry Date
LyophilizedRoom Temperature2 weeks
Reconstituted as per CofA-20°C to -80°C6 months
Reconstituted as per CofA4°C1 week
Avoid repeated freeze-thaw cycles.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile water containing 0.1% endotoxin-free recombinant human serum albumin (HSA). Gently swirl or tap vial to mix.

GMP Quality Policies

HumanKine® GMP (Good Manufacturing Practice) recombinant proteins are manufactured and validated in accordance with ISO 13485 quality management system and is compliant with GMP.
Our GMP recombinant proteins are animal component free (ACF), xeno free (XF) and tag free (TF). Read more about these policies here.

Background

FMS-related tyrosine kinase 3 ligand (FLT3 Ligand) is a growth factor that regulates proliferation of early hematopoietic cells. FLT3 Ligand binds to cells expressing the tyrosine kinase receptor FLT3. FLT3 Ligand by itself does not stimulate proliferation of early hematopoietic cells, but synergizes with other CSFs and interleukins to induce growth and differentiation (PMID: 8590775; 7544638; 12176867). GMP grade Humankine® Recombinant Human FLT3 ligand was expressed and purified tag free from HEK293 human cell line without the use of any animal components.

Synonyms

FL, Flt3 ligand, Flt3L, FLT3LG, SL cytokine