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  • HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human VEGF165 protein

Activity(Primary)

0.3-3.75 ng/mL

Species Reactivity

human,pig

Purity

>95%

Cat no : HZ-1038

Synonyms

L VEGFA, MVCD1, Vascular permeability factor, VEGF, VEGF A, VEGF165, VEGFA, VPF

Validation Data Gallery

  • Purity of recombinant human VEGF165 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue

    Purity of recombinant human VEGF165 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue

  • Recombinant human VEGF165 (HZ-1038) induces dose-dependent proliferation of the HUVEC (human umbilical vein endothelial) cell line. Cell number was quantitatively assessed by PrestoBlue® cell viability reagent. HUVEC cells were treated with increasing concentrations of recombinant VEGF165 for 96 hours. The EC50 was determined using a 4-parameter non-linear regression model. Activity determination was conducted in triplicate on a validated bioassay. The EC50 range is 0.3-3.75 ng/mL.

    Recombinant human VEGF165 (HZ-1038) induces dose-dependent proliferation of the HUVEC (human umbilical vein endothelial) cell line. Cell number was quantitatively assessed by PrestoBlue® cell viability reagent. HUVEC cells were treated with increasing concentrations of recombinant VEGF165 for 96 hours. The EC50 was determined using a 4-parameter non-linear regression model. Activity determination was conducted in triplicate on a validated bioassay. The EC50 range is 0.3-3.75 ng/mL.

Technical Specifications

GeneID 7422
Species Human
Expression HEK293
Activity(Primary) 0.3-3.75 ng/mL
Activity(Secondary)0.3-0.375
Purity >95%
Endotoxin<1 EU/µg
Accession NumberP15692
Molecular Mass 22 to 30 and 50 kDa reduced, 40 to 50 and 75 kDa non-reduced, homodimer, glycosylated
Formulation 1x PBS, See Certificate of Analysis for details
Species Reactivity human,pig

Stability and Reconstitution

Stability and StorageProduct FormTemperature ConditionsStorage Time
(From Date of Receipt)
Lyophilized-20°C to -80°CUntil Expiry Date
LyophilizedRoom Temperature2 weeks
Reconstituted as per CofA-20°C to -80°C6 months
Reconstituted as per CofA4°C1 week
Avoid repeated freeze-thaw cycles.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 1x PBS pH 7.4 containing 0.1% endotoxin-free recombinant serum albumin (HSA). Gently swirl or tap vial to mix.

Background

This gene is a member of the PDGF/VEGF growth factor family. It encodes a heparin-binding protein, which exists as a disulphide-linked homodimer (PMID 10838264; 11329058). This growth factor induces proliferation and migration of vascular endothelial cells and is essential for physiological and pathological angiogenesis. Misregulations of this gene in mice result in abnormal embryonic blood vessel formation.

Synonyms

L VEGFA, MVCD1, Vascular permeability factor, VEGF, VEGF A, VEGF165, VEGFA, VPF

Publications

SpeciesTitle
human

Nat Methods

Conversion of human fibroblasts to angioblast-like progenitor cells.

Authors - LeoKurian
View Article
human

Cell Res

Single-cell transcriptomic atlas of primate cardiopulmonary aging.

Authors - Shuai Ma
View Article
human

Circulation

Identification of novel long noncoding RNAs underlying vertebrate cardiovascular development.

Authors - LeoKurian
View Article
human

Cell Res

SIRT6 safeguards human mesenchymal stem cells from oxidative stress by coactivating NRF2.

Authors - HuizePan
View Article

Nat Commun

DutaFabs are engineered therapeutic Fab fragments that can bind two targets simultaneously.

Authors - Roland Beckmann
View Article
human

EMBO J

RhoJ integrates attractive and repulsive cues in directional migration of endothelial cells.

Authors - Yoko Fukushima
View Article