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  • HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human HGH protein

Activity(Primary)

0.05-0.5 ng/mL

Species Reactivity

human,rat

Purity

>95%

Cat no : HZ-1007

Synonyms

GH, GH N, GH1, GHN, Growth hormone, growth hormone 1, hGH N, Pituitary growth hormone, Somatotropin

Validation Data Gallery

  • Purity of recombinant human HGH was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

    Purity of recombinant human HGH was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

  • Recombinant human HGH (HZ-1007) stimulates dose-dependent proliferation of the NB211 rat lymphoma cell line. Cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. NB211 cells were treated with increasing concentrations of recombinant HGH for 96 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 0.05-0.5 ng/mL​.

    Recombinant human HGH (HZ-1007) stimulates dose-dependent proliferation of the NB211 rat lymphoma cell line. Cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. NB211 cells were treated with increasing concentrations of recombinant HGH for 96 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 0.05-0.5 ng/mL​.

  • Proteintech HGH (HZ-1007) demonstrates equivalent induction of proliferation and a 2-fold lower EC50 compared to leading competitors. Recombinant human HGH stimulates dose-dependent proliferation of the NB211 rat lymphoma cell line. Cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. NB211 cells were treated with increasing concentrations of recombinant HGH for 96 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 0.02-0.120 ng/mL​.

    Proteintech HGH (HZ-1007) demonstrates equivalent induction of proliferation and a 2-fold lower EC50 compared to leading competitors. Recombinant human HGH stimulates dose-dependent proliferation of the NB211 rat lymphoma cell line. Cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. NB211 cells were treated with increasing concentrations of recombinant HGH for 96 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 0.02-0.120 ng/mL​.

Technical Specifications

GeneID 2688
Species Human
Expression HEK293
Activity(Primary) 0.05-0.5 ng/mL
Purity >95%
Endotoxin<1 EU/μg
Accession NumberP01241
Molecular Mass 22 kDa reduced, 20 kDa non-reduced, monomer, non-glycosylated
Formulation 1x PBS, See Certificate of Analysis for details
Species Reactivity human,rat

Stability and Reconstitution

Stability and StorageProduct FormTemperature ConditionsStorage Time
(From Date of Receipt)
Lyophilized-20°C to -80°CUntil Expiry Date
LyophilizedRoom Temperature2 weeks
Reconstituted as per CofA-20°C to -80°C6 months
Reconstituted as per CofA4°C1 week
Avoid repeated freeze-thaw cycles.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 1xPBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA). Gently swirl or tap vial to mix.

Background

Human Growth hormone (HGH), also known as somatotropin, is an integral mitogen for development. It is synthesized, stored and secreted by somatotropic cells within the anterior pituitary gland (PMID 13975314). HGH stimulates growth, cell reproduction, and cell regeneration in humans and other animals (PMID 7079756). HGH stimulates both the differentiation and proliferation of myoblasts, and also stimulates amino acid uptake and protein synthesis in muscle and other tissues (PMID 8203982, 16332656).

Synonyms

GH, GH N, GH1, GHN, Growth hormone, growth hormone 1, hGH N, Pituitary growth hormone, Somatotropin

Publications

SpeciesTitle
human

J Biol Chem

Pyruvate dehydrogenase complex (PDC) subunits moonlight as interaction partners of phosphorylated STAT5 in adipocytes and adipose tissue.

Authors - Allison JRichard
View Article
rat

Protein Expr Purif

High-level expression and purification of recombinant human growth hormone produced in soluble form in Escherichia coli.

Authors - Zdenko Levarski
View Article

Adv Sci (Weinh)

Investigating Novel Therapeutic Approaches for Idiopathic Short Stature: Targeting siRNA and Growth Hormone Delivery to the Growth Plate Using Exosome Nanoparticles

Authors - Jinghong Yuan
View Article