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  • HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human HGH protein- GMP grade

EC50

0.05-0.5 ng/mL

Species Reactivity

human,rat

Purity

>95%

ISO 13485 certified HumanKine® human cell-expressed cytokines and growth factors

Cat no : HZ-1007-GMP

Synonyms

GH, GH N, GH1, GHN, Growth hormone, growth hormone 1, hGH N, Pituitary growth hormone, Somatotropin

Validation Data Gallery

  • Purity of recombinant human HGH was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

    Purity of recombinant human HGH was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

  • GMP Recombinant human HGH (HZ-1007-GMP) stimulates dose-dependent proliferation of the NB211 rat lymphoma cell line. Cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. NB211 cells were treated with increasing concentrations of GMP recombinant HGH for 96 hours. The EC50 was determined using a 4-parameter non-linear regression model. Activity determination was conducted in triplicate on a validated bioassay. The EC50 range is 0.05-0.5 ng/mL​.

    GMP Recombinant human HGH (HZ-1007-GMP) stimulates dose-dependent proliferation of the NB211 rat lymphoma cell line. Cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. NB211 cells were treated with increasing concentrations of GMP recombinant HGH for 96 hours. The EC50 was determined using a 4-parameter non-linear regression model. Activity determination was conducted in triplicate on a validated bioassay. The EC50 range is 0.05-0.5 ng/mL​.

Technical Specifications

GeneID 2688
Species Human
Expression HEK293
EC50 0.05-0.5 ng/mL
Purity >95%
Endotoxin<0.1 EU/μg
Accession NumberP01241
Molecular Mass 22 kDa reduced, 20 kDa non-reduced, monomer, non-glycosylated
Formulation 1x PBS, See Certificate of Analysis for details
Species Reactivity human,rat

Stability and Reconstitution

Stability and StorageProduct FormTemperature ConditionsStorage Time
(From Date of Receipt)
Lyophilized-20°C to -80°CUntil Expiry Date
LyophilizedRoom Temperature2 weeks
Reconstituted as per CofA-20°C to -80°C6 months
Reconstituted as per CofA4°C1 week
Avoid repeated freeze-thaw cycles.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 1xPBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA). Gently swirl or tap vial to mix.

GMP Quality Policies

HumanKine® GMP (Good Manufacturing Practice) recombinant proteins are manufactured and validated in accordance with ISO 13485 quality management system and is compliant with GMP.
Our GMP recombinant proteins are animal component free (ACF), xeno free (XF) and tag free (TF). Read more about these policies here.

Background

Human Growth hormone (HGH), also known as somatotropin, is an integral mitogen for development. It is synthesized, stored and secreted by somatotropic cells within the anterior pituitary gland (PMID 13975314). HGH stimulates growth, cell reproduction, and cell regeneration in humans and other animals (PMID 7079756). HGH stimulates both the differentiation and proliferation of myoblasts, and also stimulates amino acid uptake and protein synthesis in muscle and other tissues (PMID 8203982, 16332656).

Synonyms

GH, GH N, GH1, GHN, Growth hormone, growth hormone 1, hGH N, Pituitary growth hormone, Somatotropin