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APC Anti-Human CD19 (SJ25C1)

CD19 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

FC

Conjugate

APC Fluorescent Dye

CloneNo.

SJ25C1

Cat no : APC-65145

Synonyms

B lymphocyte antigen CD19, B4, CD19, CD19 molecule, Differentiation antigen CD19, T cell surface antigen Leu 12

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human peripheral blood lymphocytes using APC Anti-Human CD19 (SJ25C1) (APC-65145)

    FC experiment of human peripheral blood lymphocytes using APC-65145

    100 ul human peripheral blood were surface stained with FITC Anti-Human CD3 (FITC-65151, Clone: UCHT1) and 5.00 ul APC Anti-Human CD19 (APC-65145, Clone: SJ25C1). Lymphocytes were gated for analysis. Cells were not fixed.

  • Flow cytometry (FC) experiment of human peripheral blood lymphocytes using APC Anti-Human CD19 (SJ25C1) (APC-65145)

    FC experiment of human peripheral blood lymphocytes using APC-65145

    100 ul human peripheral blood were surface stained with FITC Anti-Human CD3 (FITC-65151, Clone: UCHT1) and APC Mouse IgG1 isotype control antibody. Lymphocytes were gated for analysis. Cells were not fixed.

Tested Applications

Positive FC detected inhuman peripheral blood lymphocytes

Recommended dilution

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl(0.25µg) per 10^6 cells in a 100 µl suspension or 5 µl(0.25µg) per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

APC-65145 targets CD19 in FC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen NALM1 + NALM16 cells
Full Name CD19 molecule
Calculated Molecular Weight 556 aa, 61 kDa
GenBank Accession NumberBC006338
Gene Symbol CD19
Gene ID (NCBI) 930
Conjugate APC Fluorescent Dye
Excitation/Emission Maxima Wavelengths650 nm / 660 nm
Form Liquid
Purification MethodAffinity purification
Storage Buffer Phosphate based buffer with 0.09% sodium azide and 0.1% gelatin, pH 7.2.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD19 is a 95 kDa type I transmembrane glycoprotein belonging to the immunoglobulin superfamily (PMID: 2472450). It is expressed by B cells and follicular dendritic cells. CD19 is up-regulated at the step of B-lineage commitment during the differentiation of the hematopoietic stem cell, it remains on during subsequent stages of differentiation until finally down-regulated during terminal differentiation into plasma cells (PMID: 8528044). CD19 is involved in B cell development, activation and differentiation. It is the dominant component for the signaling complex on B cells that includes CD21 (CR2), CD81 (TAPA-1) and CD225 and acts as a critical co-receptor for BCR signal transduction (PMID: 23210908).

Protocols

Product Specific Protocols
FC protocol for APC CD19 antibody APC-65145Download protocol
Standard Protocols
Click here to view our Standard Protocols