FC experiment of mouse bone marrow cells using 98197-1-RR
1x10^6 mouse bone marrow cells were intracellularly stained with 0.25 ug Anti-Mouse Neutrophil elastase Rabbit Recombinant Antibody (98197-1-RR, Clone: 241842G3) (red) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
1x10^6 mouse bone marrow cells were intracellularly stained with 0.25 ug Anti-Mouse Neutrophil elastase Rabbit Recombinant Antibody (98197-1-RR, Clone: 241842G3) (red) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
FC experiment of mouse bone marrow cells using 98197-1-RR
1x10^6 mouse bone marrow cells were intracellularly stained with 0.25 ug Anti-Mouse Neutrophil elastase Rabbit Recombinant Antibody (98197-1-RR, Clone: 241842G3) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
1x10^6 mouse bone marrow cells were intracellularly stained with 0.25 ug Anti-Mouse Neutrophil elastase Rabbit Recombinant Antibody (98197-1-RR, Clone: 241842G3) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
Affinity and Kinetic Characterization of 98197-1-RR
Biolayer interferometry (BLl) kinetic assays of 98197-1-RR against Mouse Neutrophil elastase were performed. The affinity constant is below 1 pM.
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This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Product Information
98197-1-RR targets Neutrophil elastase in FC (Intra) applications and shows reactivity with mouse samples.
FC experiment of mouse bone marrow cells using 98197-1-RR
1x10^6 mouse bone marrow cells were intracellularly stained with 0.25 ug Anti-Mouse Neutrophil elastase Rabbit Recombinant Antibody (98197-1-RR, Clone: 241842G3) (red) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
FC experiment of mouse bone marrow cells using 98197-1-RR
1x10^6 mouse bone marrow cells were intracellularly stained with 0.25 ug Anti-Mouse Neutrophil elastase Rabbit Recombinant Antibody (98197-1-RR, Clone: 241842G3) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
AFFINITY Figures
Affinity and Kinetic Characterization of 98197-1-RR
Biolayer interferometry (BLl) kinetic assays of 98197-1-RR against Mouse Neutrophil elastase were performed. The affinity constant is below 1 pM.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
Neutrophil elastase Recombinant antibody
Catalog Number
98197-1-RR
Citations
-
Dilutions
FC (INTRA) :
Applications
FC (Intra)
Reactivity
mouse
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
