• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

Anti-Human CXCR4/CD184 Rabbit Recombinant Antibody

CXCR4/CD184 Uni-rAbTM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

241735F8

Cat no : 98175-1-RR

Synonyms

CXCR4, CXCR 4, CXC R4, C-X-C chemokine receptor type 4, CD184

Validation Data Gallery

Filter:
  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CXCR4/CD184 Rabbit Recombinant Antibody (98175-1-RR)

    FC experiment of human PBMCs using 98175-1-RR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CXCR4/CD184 Rabbit Recombinant Antibody (98175-1-RR, Clone: 241735F8),or Isotype Control, and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with CoraLite® Plus 647 Anti-Human CD19. Cells were not fixed. Lymphocytes were gated.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CXCR4/CD184 Rabbit Recombinant Antibody (98175-1-RR)

    FC experiment of human PBMCs using 98175-1-RR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CXCR4/CD184 Rabbit Recombinant Antibody (98175-1-RR, Clone:241735F8)(red) or Isotype Control (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. Lymphocytes were gated.

Tested Applications

Positive FC detected inhuman PBMCs

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

98175-1-RR targets CXCR4/CD184 in FC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant Protein
Full Name chemokine (C-X-C motif) receptor 4
Calculated Molecular Weight 352 aa, 40 kDa
GenBank Accession NumberBC020968
Gene Symbol CXCR4
Gene ID (NCBI) 7852
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.09% sodium azide, pH 7.3.
Storage ConditionsStore at 2 - 8°C. Stable for one year after shipment.

Background Information

C-X-C chemokine receptor type 4 (CXCR4, also known as CD184) is a widely expressed G protein-coupled seven-transmembrane receptor. CXCL12/SDF-1 is the biological ligand for CXCR4. The binding of CXCL12 to CXCR4 induces intracellular signaling through several divergent pathways initiating signals related to chemotaxis, cell survival and/or proliferation, increase in intracellular calcium, and gene transcription (PMID: 20484021). CXCR4 also functions as a coreceptor for HIV-1 entry (PMID: 9427609).

Protocols

Product Specific Protocols
FC protocol for CXCR4/CD184 antibody 98175-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols