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Anti-Human TWEAKR/CD266 Rabbit Recombinant Antibody

CD266 Uni-rAbTM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

240991C5

Cat no : 98125-1-RR

Synonyms

TNFRSF12A, TweakR, FGF-inducible 14, FGF inducible 14, DR3LG

Validation Data Gallery

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  • Flow cytometry (FC) experiment of HT-1080 cells using Anti-Human CD266 Rabbit Recombinant Antibody (98125-1-RR)

    FC experiment of HT-1080 using 98125-1-RR

    1x10^6 HT-1080 cells were surface stained with 0.25 ug Anti-Human TWEAKR Rabbit Recombinant Antibody (98125-1-RR, Clone: 240991C5) (red) or 0.25 ug Isotype Control (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

  • Affinity and Kinetic Characterization of 98125-1-RR

    Affinity and Kinetic Characterization of 98125-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98125-1-RR against Human CD266 were performed. The affinity constant is 0.508 nM.

Tested Applications

Positive FC detected inHT-1080 cells

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 98125-1-RR is CD266 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CD266 fusion protein Ag13823
Full Name tumor necrosis factor receptor superfamily, member 12A
Calculated Molecular Weight 129 aa, 14 kDa
GenBank Accession NumberBC002718
Gene Symbol TWEAKR
Gene ID (NCBI) 51330
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.09% sodium azide, pH 7.3.
Storage ConditionsStore at 2 - 8°C. Stable for one year after shipment.

Background Information

TweakR (Fn14, TNFRSF12A) is a member of the TNF receptor superfamily which is activated by its ligand, the cytokine TWEAK (TNFSF12). TweakR is the smallest member of the TNFR superfamily . TweakR was first described as an FGF-inducible gene which played a role in fibroblast adhesion and migration . Subsequently, the induction of TweakR expression by other growth factors and/or upon tissue injury was observed in multiple cell types, including hepatocytes, endothelial cells, adipocytes, and cardiomyocytes. (PMID: 23073510, PMID: 24409185)

Protocols

Product Specific Protocols
FC protocol for CD266 antibody 98125-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols