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Anti-Human CD22 Rabbit Recombinant Antibody

CD22 Uni-rAbTM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

241315A1

Cat no : 98092-1-RR

Synonyms

CD22 molecule, B-lymphocyte cell adhesion molecule, BL-CAM, B-cell receptor CD22, B cell receptor CD22

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD22 Rabbit Recombinant Antibody (98092-1-RR)

    FC experiment of human PBMCs using 98092-1-RR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD22 Rabbit Recombinant Antibody (98092-1-RR, Clone:241315A1) or Isotype Control and PE-conjugated Goat Anti-Rabbit IgG, Cells were then stained with CoraLite® Plus 647 Anti-Human CD19 (4G7) Mouse IgG2a Recombinant Antibody (CL647-65562, Clone: 4G7). Cells were not fixed.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD22 Rabbit Recombinant Antibody (98092-1-RR)

    FC experiment of human PBMCs using 98092-1-RR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD22 Rabbit Recombinant Antibody (98092-1-RR, Clone:241315A1) or Isotype Control and PE-conjugated Goat Anti-Rabbit IgG. Cells were not fixed.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD22 Rabbit Recombinant Antibody (98092-1-RR)

    FC experiment of human PBMCs using 98092-1-RR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD22 Rabbit Recombinant Antibody (98092-1-RR, Clone:241315A1) and PE-conjugated Goat Anti-Rabbit IgG, and 0.25 ug Isotype Control, and 0.25 ug CoraLite® Plus 647 Anti-Human CD19 (4G7) Mouse IgG2a Recombinant Antibody (CL647-65562, Clone: 4G7). Cells were not fixed.

  • Affinity and Kinetic Characterization of 98092-1-RR

    Affinity and Kinetic Characterization of 98092-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98092-1-RR against Human CD22 were performed. The affinity constant is 3.11 nM.

Tested Applications

Positive FC detected inhuman PBMCs

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

98092-1-RR targets CD22 in FC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant Protein
Full Name CD22 molecule
Calculated Molecular Weight 847 aa, 95 kDa
GenBank Accession NumberBC109306
Gene Symbol CD22
Gene ID (NCBI) 933
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.09% sodium azide, pH 7.3.
Storage ConditionsStore at 2 - 8°C. Stable for one year after shipment.

Background Information

CD22, also known as Siglec-2 (sialic acid binding Ig-like lectin 2) or BL-CAM (B-lymphocyte cell adhesion molecule), is a 130-140 kDa, B-cell restricted, type I transmembrane glycoprotein belonging to the immunoglobulin gene superfamily. The expression of CD22 is developmentally regulated. It is expressed at low levels in the cytoplasm of pro-B and pre-B cells and present on the cell surface only at mature stages of B-cell differentiation. Cell surface expression is lost during terminal differentiation into plasma cell and after B-cell activation. CD22 is an inhibitory receptor for B-cell receptor (BCR) signalling, preferentially binds to alpha-2,6-linked sialic acid and mediates B-cell B-cell interactions. It plays a crucial role in activation and differentiation of the B-cell.

Protocols

Product Specific Protocols
FC protocol for CD22 antibody 98092-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols