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Anti-Human IL-7Ra/CD127 Rabbit Recombinant Antibody

IL-7Ra/CD127 Uni-rAbTM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

240749H2

Cat no : 98074-1-RR

Synonyms

CD127, 240749H2, IL 7RA, IL 7R subunit alpha, IL 7R alpha

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human IL-7Ra/CD127 Rabbit Recombinant Antibod (98074-1-RR)

    FC experiment of human PBMCs using 98074-1-RR

    1x10^6 human PBMCs were stained with CoraLite® Plus 647 Anti-Human CD3 (OKT3) Mouse IgG2a Recombinant Antibody (CL647-65569, Clone: OKT3) and 0.25 ug Anti-Human IL-7Ra/CD127 Rabbit Recombinant Antibody (98074-1-RR, Clone:240749H2), or Isotype Control. Cells were not fixed.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human IL-7Ra/CD127 Rabbit Recombinant Antibod (98074-1-RR)

    FC experiment of human PBMCs using 98074-1-RR

    1x10^6 human PBMCs were stained with 0.25 ug Anti-Human IL-7Ra/CD127 Rabbit Recombinant Antibody (98074-1-RR, Clone:240749H2) (red), or Isotype Control(blue). Cells were not fixed.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human IL-7Ra/CD127 Rabbit Recombinant Antibod (98074-1-RR)

    FC experiment of human PBMCs using 98074-1-RR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human IL-7Ra/CD127 Rabbit Recombinant Antibody (98074-1-RR, Clone:240749H2) and PE-Conjugated Goat Anti-Rabbit IgG(H+L), and 0.25 ug CoraLite® Plus 647 Anti-Human CD3 (OKT3) Mouse IgG2a Recombinant Antibody (CL647-65569, Clone: OKT3), and 0.25 ug Isotype Control. Cells were not fixed.

  • Affinity and Kinetic Characterization of 98074-1-RR

    Affinity and Kinetic Characterization of 98074-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98074-1-RR against Human IL-7Ra/CD127 were performed. The affinity constant is 4.57 nM.

Tested Applications

Positive FC detected inhuman PBMCs

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 98074-1-RR is IL-7Ra/CD127 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein
Full Name interleukin 7 receptor
Calculated Molecular Weight52 kDa
GenBank Accession NumberAAC83204.1
Gene Symbol CD127
Gene ID (NCBI) 3575
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.09% sodium azide, pH 7.3.
Storage ConditionsStore at 2 - 8°C. Stable for one year after shipment.

Background Information

CD127, also known as IL-7R subunit alpha (IL-7RA), is a type I membrane glycoprotein expressed on thymocytes, B cell precursors, most T cells, and some lymphoid and myeloid cells. IL-7R is a heterodimer composed of CD127 and the common-γ chain receptor (CD132). IL-7R plays critical roles in lymphocyte development and homeostasis. CD127 can also act as a receptor for thymic stromal lymphopoietin (TSLP).

Protocols

Product Specific Protocols
FC protocol for IL-7Ra/CD127 antibody 98074-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols