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Anti-Human CD86 Rabbit Recombinant Antibody

CD86 Uni-rAbTM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

FC

Conjugate

Unconjugated

CloneNo.

240429C6

Cat no : 98043-1-RR

Synonyms

BU63, B70, B7 2, Activation B7-2 antigen, Activation B7 2 antigen

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD86 Rabbit Recombinant Antibody (98043-1-RR)

    FC experiment of human PBMCs using 98043-1-RR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD86 Rabbit Recombinant Antibody (98043-1-RR, Clone: 240429C6) or 0.25 ug Isotype Control and PE-conjugated Goat Anti-Rabbit IgG. Cells were then stained with APC Anti-Human CD14. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD86 Rabbit Recombinant Antibody (98043-1-RR)

    FC experiment of human PBMCs using 98043-1-RR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD86 Rabbit Recombinant Antibody (98043-1-RR, Clone: 240429C6) (red) or 0.25 ug Isotype Control (blue) and PE-conjugated Goat Anti-Rabbit IgG. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • Affinity and Kinetic Characterization of 98043-1-RR

    Affinity and Kinetic Characterization of 98043-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98043-1-RR against Human CD86 were performed. The affinity constant is 0.23 nM.

Tested Applications

Positive FC detected inhuman PBMCs

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 98043-1-RR is CD86 Fusion Protein expressed in E. coli.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein
Full Name CD86 molecule
Calculated Molecular Weight 329 aa, 38 kDa
GenBank Accession NumberNM_175862
Gene Symbol CD86
Gene ID (NCBI) 942
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

Background Information

CD86 (also known as B7-2) is a costimulatory molecule belonging to the immunoglobulin superfamily. Primarily expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, and macrophages, CD86 is the ligand for two proteins at the cell surface of T cells, CD28 antigen and CTLA-4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with CTLA-4 negatively regulates T-cell activation and diminishes the immune response. (PMID: 7513726; 1847722; 11029388; 27591335)

Protocols

Product Specific Protocols
FC protocol for CD86 antibody 98043-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols