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Anti-Mouse IL-15 Rabbit Recombinant Antibody

IL-15 Uni-rAbTM Recombinant Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC (Intra)

Conjugate

Unconjugated

CloneNo.

230285B3

Cat no : 98036-1-RR

Synonyms

Il15, interleukin 15, AI503618, 230285B3

Validation Data Gallery

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  • Flow cytometry (FC) experiment of Mouse PBMCs using Anti-Mouse IL-15 Rabbit Recombinant Antibody (98036-1-RR)

    FC experiment of Mouse PBMCs using 98036-1-RR

    1x10^6 Mouse PBMCs were intracellularly stained with 0.25 ug Anti-Mouse IL-15 Rabbit Recombinant Antibody (98036-1-RR, Clone: 230285B3) (red) or Isotype Control (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Intracellular Staining Permeabilization Wash Buffer.

  • Affinity and Kinetic Characterization of 98036-1-RR

    Affinity and Kinetic Characterization of 98036-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98036-1-RR against Mouse IL-15 were performed. The affinity constant is below 1 pM.

Tested Applications

Positive FC (Intra) detected inMouse PBMCs

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 98036-1-RR is IL-15 Fusion Protein expressed in E. coli.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein
Full Name interleukin 15
Calculated Molecular Weight 19 kDa
GenBank Accession NumberNM_001254747
Gene Symbol IL-15
Gene ID (NCBI) 16168
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

Background Information

IL-15 is a 4-α-helix bundle cytokine playing a pivotal role in stimulation of both innate and adaptive immune cells. It is produced primarily by keratinocytes, skeletal muscle cells, monocytes, and CD4+ T cells. It is a member of the common gamma chain family. The members of the common gamma chain family include the IL-2, IL-4, IL-7, IL-9, and IL-21 and require binding to the common gamma chain receptor for activation. It can be used in growth and maintenance of T and NK cells. It is also shown to be used in proliferation and functional effect of T cells for adoptive cell therapy. It is a glycosylated protein and HumanKine IL-15 appear as 12.5-25 kDa bands (PMID: 24587813, 26627006, 27849617, 31250350)