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  • Western Blot (WB) analysis of human peripheral blood platelets using CD40L/CD154 Recombinant antibody (85209-4-RR)

    WB analysis of human peripheral blood platelets using 85209-4-RR

    human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 85209-4-RR (CD40L/CD154 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

CD40L/CD154 Recombinant antibody

CD40L/CD154 Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 85209-4-RR
Clone No.242847A5

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

CD154, CD40L; CD154, CD40LG, CD40 ligand, CD40 L

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive WB detected inhuman peripheral blood platelets

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85209-4-RR targets CD40L/CD154 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein Predict reactive species
Full Name CD40 ligand
Calculated Molecular Weight 261 aa, 29 kDa
Observed Molecular Weight30 kDa
GenBank Accession NumberBC071754
Gene Symbol CD40 Ligand
Gene ID (NCBI) 959
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP29965
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The CD40 ligand (CD40L, TRAP, CD154), a member of the TNF superfamily of ligands, is expressed as either a 33 kDa transmembrane homologue or an 18 kDa soluble form (sCD154). CD40L is primarily expressed on activated CD4+ T cells and a small proportion of CD8+ T cells and platelets. It binds to CD40 on antigen-presenting cells (APC), which leads to many effects depending on the target cell type. Recent studies have suggested that CD40/CD40L interactions regulate oxidative stress and affect various signaling pathways in both the immunological and the cardiovascular systems. The CD40/CD40L system is also involved in tumorigenesis.

Protocols

Product Specific Protocols
WB protocol for CD40L/CD154 antibody 85209-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols