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  • Immunofluorescence (IF) / fluorescent staining of T-47D cells using INPP4B Recombinant antibody (85151-1-RR)

    IF Staining of T-47D using 85151-1-RR

    Immunofluorescent analysis of (4% PFA) fixed T-47D cells using INPP4B antibody (85151-1-RR, Clone: 242837A4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of K562 using INPP4B Recombinant antibody (85151-1-RR)

    FC experiment of K562 using 85151-1-RR

    1x10^6 K562 were intracellularly stained with 0.25 ug INPP4B Recombinant antibody (85151-1-RR, Clone:242837A4) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 85151-1-RR

    Affinity and Kinetic Characterization of 85151-1-RR

    Biolayer interferometry (BLl) kinetic assays of 85151-1-RR against Human INPP4B were performed. The affinity constant is 0.52 nM.

INPP4B Recombinant antibody

INPP4B {ptg:Brand} Recombinant Antibody for IF/ICC, FC (Intra), ELISA
Cat No. 85151-1-RR
Clone No.242837A4

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Synonyms

242837A4, EC:3.1.3.66, Inositol polyphosphate 4-phosphatase type II, Type II inositol 3,4-bisphosphate 4-phosphatase

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive IF/ICC detected inT-47D cells
Positive FC (Intra) detected inK562

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85151-1-RR targets INPP4B in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen INPP4B fusion protein Ag8676 Predict reactive species
Full Name inositol polyphosphate-4-phosphatase, type II, 105kDa
Calculated Molecular Weight 53 aa, 6 kDa
GenBank Accession NumberBC005273
Gene Symbol INPP4B
Gene ID (NCBI) 8821
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO15327
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

INPP4B (Inositol polyphosphate 4-phosphatase type II) can catalyze the hydrolysis of the 4-position phosphate of phosphatidylinositol 3,4-bisphosphate, inositol 1,3,4-trisphosphate and inositol 3,4-trisphosphate (PMID: 24070612; 24591580). It also Plays a role in the late stages of macropinocytosis by dephosphorylating phosphatidylinositol 3,4-bisphosphate in membrane ruffles (PMID: 24591580).

Protocols

Product Specific Protocols
IF protocol for INPP4B antibody 85151-1-RRDownload protocol
FC protocol for INPP4B antibody 85151-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols