Tested Applications
Positive WB detected in | HeLa cells, HepG2 cells, HEK-293T cells, Jurkat cells, A431 cells |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:5000-1:50000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
85148-1-RR targets RFC1 in WB, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | RFC1 fusion protein Ag13585 Predict reactive species |
Full Name | replication factor C (activator 1) 1, 145kDa |
Calculated Molecular Weight | 128 kDa |
Observed Molecular Weight | 128-150 kDa |
GenBank Accession Number | BC051751 |
Gene Symbol | RFC1 |
Gene ID (NCBI) | 5981 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P35251 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Replication factor C subunit 1 (RFC1) is a pentameric DNA polymerase accessory protein involved in DNA replication and repair (PMID: 10051561). It was reported as a cause of cerebellar ataxia with neuropathy and vestibular areflexia syndrome (CANVAS) (PMID: 30926972).
Protocols
Product Specific Protocols | |
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WB protocol for RFC1 antibody 85148-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |