• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
Filter:
  • Western Blot (WB) analysis of various lysates using AKAP1 Recombinant antibody (85119-2-RR)

    WB analysis using 85119-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85119-2-RR (AKAP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using AKAP1 Recombinant antibody (85119-2-RR)

    IF Staining of HepG2 using 85119-2-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using AKAP1 antibody (85119-2-RR, Clone: 242763A5 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using AKAP1 Recombinant antibody (85119-2-RR)

    IF Staining of MCF-7 using 85119-2-RR

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using AKAP1 antibody (85119-2-RR, Clone: 242763A5 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of HEK-293T cells using AKAP1 Recombinant antibody (85119-2-RR)

    FC experiment of HEK-293T using 85119-2-RR

    1x10^6 HEK-293T cells were intracellularly stained with 0.13 ug AKAP1 Recombinant antibody (85119-2-RR, Clone:242763A5) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.13 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

AKAP1 Recombinant antibody

AKAP1 {ptg:Brand} Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 85119-2-RR
Clone No.242763A5

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

242763A5, AKAP, AKAP 1, A-kinase anchor protein 1, mitochondrial, A-kinase anchor protein 149 kDa

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

Please visit your regions distributor:

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, HepG2 cells, MCF-7 cells, PC-3 cells, Caco-2 cells
Positive IF/ICC detected inHepG2 cells, MCF-7 cells
Positive FC (Intra) detected inHEK-293T cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85119-2-RR targets AKAP1 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen AKAP1 fusion protein Ag8037 Predict reactive species
Full Name A kinase (PRKA) anchor protein 1
Calculated Molecular Weight 97 kDa
Observed Molecular Weight149 kDa
GenBank Accession NumberBC000729
Gene Symbol AKAP1
Gene ID (NCBI) 8165
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ92667
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

AKAP1 (also termed AKAP149) is a human 149 kDa anchoring protein localized in mitochondria and the endoplasmic reticulum/nuclear envelope (ER-NE) network. It Binds to type I and II regulatory subunits of protein kinase A and anchors them to the cytoplasmic face of the mitochondrial outer membrane. This protein is speculated to be involved in the cAMP-dependent signal transduction pathway and in directing RNA to a specific cellular compartment.

Protocols

Product Specific Protocols
WB protocol for AKAP1 antibody 85119-2-RRDownload protocol
IF protocol for AKAP1 antibody 85119-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols