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  • Western Blot (WB) analysis of various lysates using GOSR2/Membrin Recombinant antibody (84962-4-RR)

    WB analysis using 84962-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84962-4-RR (GOSR2/Membrin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using GOSR2/Membrin Recombinant antibody (84962-4-RR)

    IF Staining of HeLa using 84962-4-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GOSR2/Membrin antibody (84962-4-RR, Clone: 242589D8 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

GOSR2/Membrin Recombinant antibody

GOSR2/Membrin Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 84962-4-RR

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

Membrin, GS27, GOSR2, Golgi SNAP receptor complex member 2, 27 kDa Golgi SNARE protein

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

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Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, Jurkat cells, L02 cells, mouse liver tissue, rat liver tissue, human placenta tissue
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84962-4-RR targets GOSR2/Membrin in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen GOSR2/Membrin fusion protein Ag2736 Predict reactive species
Full Name golgi SNAP receptor complex member 2
Calculated Molecular Weight 212 aa, 25 kDa
Observed Molecular Weight 23-25 kDa
GenBank Accession NumberBC009710
Gene Symbol Membrin
Gene ID (NCBI) 9570
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDO14653
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Golgi SNAP receptor complex member 2 (GOSR2), also known as GS27 or Membrin, is a member of the soluble NSF attachment protein receptor (SNARE) family of vesicle docking proteins. GOSR2 is localized in the Golgi apparatus. It is involved in the transport of proteins from the cis/medial-Golgi to the trans-Golgi network.

Protocols

Product Specific Protocols
WB protocol for GOSR2/Membrin antibody 84962-4-RRDownload protocol
IF protocol for GOSR2/Membrin antibody 84962-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols