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CHKA Recombinant antibody, PBS Only (Detector)

CHKA Uni-rAbTM Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

242095B4

Cat no : 84881-2-PBS

Synonyms

EC:2.7.1.32, CKI, choline kinase alpha, CHK, CHETK-alpha

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CHKA Recombinant antibody (84881-2-RR)

    WB analysis using 84881-2-RR (same clone as 84881-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84881-2-RR (CHKA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84881-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01644-2

    Cytometric bead array standard curve of MP01644-2

    Cytometric bead array standard curve of MP01644-2, CHKA Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84881-1-PBS. Detection antibody: 84881-2-PBS. Standard: Ag4761. Range: 1.563-200 ng/mL.

  • Affinity and Kinetic Characterization of 84881-2-RR

    Affinity and Kinetic Characterization of 84881-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84881-2-RR against Human CHKA were performed. The affinity constant is below 1 pM.

Product Information

84881-2-PBS targets CHKA as part of a matched antibody pair:

MP01644-2: 84881-1-PBS capture and 84881-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CHKA fusion protein Ag4761
Full Name choline kinase alpha
Calculated Molecular Weight 439 aa, 52 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC036471
Gene Symbol CHKA
Gene ID (NCBI) 1119
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Choline kinase alpha, also named as CHK, CKI and CK, belongs to the choline/ethanolamine kinase family. CHKa may have a regulatory role in phosphatidylcholine synthesis. CHKA catalyze the reaction: ATP + choline = ADP + O-phosphocholine. The human enzyme resembled the rat liver enzyme over the entire sequence. It also resembled the yeast enzyme in the carboxy-terminal region, but not in the amino-terminal region(PMID:1618328).And it expresses in human brain tissue but not in human skeletal muscle(PMID:21665002).It has 2 isoforms produced by alternative splicing.