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PISD Recombinant antibody, PBS Only (Detector)

PISD Uni-rAbTM Recombinant Antibody for WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

242280C9

Cat no : 84866-2-PBS

Synonyms

PSDC, Phosphatidylserine decarboxylase proenzyme, mitochondrial, Phosphatidylserine decarboxylase beta chain, Phosphatidylserine decarboxylase alpha chain, EC:4.1.1.65

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PISD Recombinant antibody (84866-2-RR)

    WB analysis using 84866-2-RR (same clone as 84866-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84866-2-RR (PISD antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84866-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using PISD Recombinant antibody (84866-2-RR)

    WB analysis using 84866-2-RR (same clone as 84866-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84866-2-RR (PISD antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84866-2-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP01640-2

    Sandwich ELISA standard curve of MP01640-2

    Sandwich ELISA standard curve of MP01640-2, Human PISD Recombinant Matched Antibody Pair - PBS only. 84866-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag9499. 84866-2-PBS was HRP conjugated as the detection antibody. Range: 0.391-25 ng/mL.

  • Sample test of MP01640-2

    Sample test of MP01640-2

    The mean PISD concentration was determined to be 19.97 ng/mL in L02 cell extract based on a 1.30 mg/mL extract load.

  • Cytometric bead array standard curve of MP01640-1

    Cytometric bead array standard curve of MP01640-1

    Cytometric bead array standard curve of MP01640-1, PISD Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84866-1-PBS. Detection antibody: 84866-2-PBS. Standard: Ag9499. Range: 0.781-50 ng/mL.

Product Information

84866-2-PBS targets PISD as part of a matched antibody pair:

MP01640-1: 84866-1-PBS capture and 84866-2-PBS detection (validated in Cytometric bead array)

MP01640-2: 84866-3-PBS capture and 84866-2-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen PISD fusion protein Ag9499
Full Name phosphatidylserine decarboxylase
Calculated Molecular Weight375aa,43 kDa; 408aa,46 kDa
Observed Molecular Weight46 kDa
GenBank Accession NumberBC001482
Gene Symbol PISD
Gene ID (NCBI) 23761
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

PISD(Phosphatidylserine decarboxylase proenzyme) belongs to the phosphatidylserine decarboxylase family.It contains a conserved LGST motif that functions as an autocatalytic cleavage site where the proenzyme is split into mature alpha and beta subunits.It has 2 isoforms produced by alternative splicing.