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TLR6 Recombinant antibody, PBS Only (Detector)

TLR6 Uni-rAbTM Recombinant Antibody for IF-P, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF-P, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

241926F2

Cat no : 84811-2-PBS

Synonyms

Toll-like receptor 6, toll like receptor 6, CD286, 241926F2

Validation Data Gallery

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  • Sandwich ELISA standard curve of MP01727-1

    Sandwich ELISA standard curve of MP01727-1

    Sandwich ELISA standard curve of MP01727-1, Human TLR6 Recombinant Matched Antibody Pair - PBS only. 84811-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard SY02306. 84811-2-PBS was HRP conjugated as the detection antibody. Range: 6.25-400 pg/mL.

  • Sample test of MP01727-1

    Sample test of MP01727-1

    The mean TLR6 concentration was determined to be 166.5 pg/mL in Daudi cell extract based on a 1.8 mg/mL extract load.

  • Immunofluorescence (IF) / fluorescent staining of mouse spleen tissue using TLR6 Recombinant antibody (84811-2-RR)

    IF Staining of mouse spleen using 84811-2-RR (same clone as 84811-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using TLR6 antibody (84811-2-RR, Clone: 241926F2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84811-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse spleen tissue using TLR6 Recombinant antibody (84811-2-RR)

    IF Staining of mouse spleen using 84811-2-RR (same clone as 84811-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using TLR6 antibody (84811-2-RR, Clone: 241926F2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84811-2-PBS in a different storage buffer formulation.

Product Information

84811-2-PBS targets TLR6 as part of a matched antibody pair:

MP01727-1: 84811-3-PBS capture and 84811-2-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide
Full Name toll-like receptor 6
Calculated Molecular Weight 480 aa, 56 kDa
GenBank Accession NumberBC111755
Gene Symbol TLR6
Gene ID (NCBI) 10333
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

TLR6, also known as CD286, belongs to the Toll-like receptor (TLR) family which is important in the innate immune response to pathogens. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. TLR6 can form a heterodimer with TLR2. TLR6 and TLR2 are both recruited to the macrophage phagosome, where they recognize peptidoglycan, a Gram-positive pathogen component (PMID: 11095740). TLR6 interacts with CD36, following CD36 stimulation by oxLDL or amyloid-beta 42, and forms a heterodimer with TLR4. The trimeric complex is internalized and triggers an inflammatory response.