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HMGCL Recombinant antibody

HMGCL Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

242224F9

Cat no : 84769-4-RR

Synonyms

HMG-CoA lyase, HMG CoA lyase, HL, EC:4.1.3.4, 242224F9

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HMGCL Recombinant antibody (84769-4-RR)

    WB analysis using 84769-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84769-4-RR (HMGCL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of HeLa cells using HMGCL Recombinant antibody (84769-4-RR)

    FC experiment of HeLa using 84769-4-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug HMGCL Recombinant antibody (84769-4-RR, Clone:242224F9) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 84769-4-RR

    Affinity and Kinetic Characterization of 84769-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84769-4-RR against Human HMGCL were performed. The affinity constant is 1.13 nM.

Tested Applications

Positive WB detected inmouse liver tissue, rat liver tissue, mouse kidney tissue, rat kidney tissue
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84769-4-RR targets HMGCL in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen HMGCL fusion protein Ag10426
Full Name 3-hydroxymethyl-3-methylglutaryl-Coenzyme A lyase
Calculated Molecular Weight 325 aa, 34 kDa
Observed Molecular Weight 32 kDa
GenBank Accession NumberBC010570
Gene Symbol HMGCL
Gene ID (NCBI) 3155
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HMGCL (3-hydroxy-3-methylglutaryl-CoA lyase) is a key enzyme in ketogenesis (ketone body formation). It is a ketogenic enzyme in the liver that catalyzes the formation of acetoacetate from HMG-CoA within the mitochondria. It also plays a prominent role in the catabolism of the amino acid leucine.

Protocols

Product Specific Protocols
WB protocol for HMGCL antibody 84769-4-RRDownload protocol
FC protocol for HMGCL antibody 84769-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols