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  • Western Blot (WB) analysis of NIH/3T3 cells using IL-4 R alpha/CD124 Recombinant antibody (84758-5-RR)

    WB analysis of NIH/3T3 using 84758-5-RR

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 84758-5-RR (IL-4 R alpha/CD124 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

IL-4 R alpha/CD124 Recombinant antibody

IL-4 R alpha/CD124 {ptg:Brand} Recombinant Antibody for WB, ELISA
Cat No. 84758-5-RR
Clone No.242262E3

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, ELISA

Synonyms

Il4ra, IL-4R subunit alpha, Il4r, IL-4 receptor subunit alpha, CD124

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

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Tested Applications

Positive WB detected inNIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84758-5-RR targets IL-4 R alpha/CD124 in WB, ELISA applications and shows reactivity with mouse samples.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein Predict reactive species
Full Name interleukin 4 receptor, alpha
Calculated Molecular Weight88 kDa
Observed Molecular Weight130 kDa
GenBank Accession NumberNM_001008700.3
Gene Symbol Il4ra
Gene ID (NCBI) 16190
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP16382-1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IL-4 receptor alpha chain (IL-4RA), also known as CD124, is a transmembrane glycoprotein expressed on a wide variety of hematopoietic and non-hematopoietic cells. It is a receptor for both IL-4 and IL-13. IL-4RA can complex with the common gamma chain (IL-2R gamma or CD132) and utilizes JAK1 and JAK2 for signal transduction, while complexes of IL-4RA with IL-13RA1 subunit mediate signals via JAK2 and Tyk2. The IL-4 response is involved in promoting Th2 differentiation. The IL-4/IL-13 responses are involved in regulating IgE production and, chemokine and mucus production at sites of allergic inflammation.

Protocols

Product Specific Protocols
WB protocol for IL-4 R alpha/CD124 antibody 84758-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols