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FOXM1 Recombinant antibody

FOXM1 Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

242077A7

Cat no : 84681-1-RR

Synonyms

HNF-3/fork-head homolog 11, HFH-11, HFH11, Forkhead-related protein FKHL16, FKHL16

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FOXM1 Recombinant antibody (84681-1-RR)

    WB analysis using 84681-1-RR

    A431 cells were subjected to SDS PAGE followed by western blot with 84681-1-RR (FOXM1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using FOXM1 Recombinant antibody (84681-1-RR)

    WB analysis using 84681-1-RR

    L02 cells were subjected to SDS PAGE followed by western blot with 84681-1-RR (FOXM1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84681-1-RR

    Affinity and Kinetic Characterization of 84681-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84681-1-RR against Human FOXM1 were performed. The affinity constant is 10.7 pM.

Tested Applications

Positive WB detected inL02 cells, A431 cells, HT-29 cells, U2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84681-1-RR targets FOXM1 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen FOXM1 fusion protein Ag3729
Full Name forkhead box M 1
Calculated Molecular Weight 83 kDa
Observed Molecular Weight 84-110 kDa
GenBank Accession NumberBC006192
Gene Symbol FOXM1
Gene ID (NCBI) 2305
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Forkhead box M1 (FOXM1), also named as HNF-3, HFH-11 or Trident, belongs to a superfamily of Fox transcription factors. FOXM1 is strongly associated with cell proliferation, it plays a important role in cell cycle progression by regulating both G1/S and G2/M phases, it is also involved in DNA damage repair, angiogenesis, apoptosis and tissue regeneration. Recently, crucial roles of this transcription factor were found in the development and progression of many cancers including colorectal, lung, prostate, liver and breast cancer. FOXM1 levels are significantly higher and associated with tumor grade in most human tumors, rendering it a potential target of cancer diagnosis and therapies. Catalog#13147-1-AP is a rabbit polyclonal antibody raised against C-terminal of human FOXM1. And this antibody can recognize three kinds of FOXM1, FOXM1-pp(~95-110kDa), FOXM1-B(isoform2,~83kDa) and FOXM1A(isoform4, ~90kDa). Sometimes a molecular weight of about 65 kDa can also be observed, which may be a degradation form of FOXM1 (PMID: 26547933).

Protocols

Product Specific Protocols
WB protocol for FOXM1 antibody 84681-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols