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CEBPA Recombinant antibody

CEBPA Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

242101C9

Cat no : 84668-4-RR

Synonyms

CEBP alpha, CEBP Alpha/CEBPA, CEBP, CCAAT/enhancer-binding protein alpha, C/EBPα

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CEBPA Recombinant antibody (84668-4-RR)

    IF Staining of HeLa using 84668-4-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CEBPA antibody (84668-4-RR, Clone: 242101C9 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CEBPA Recombinant antibody (84668-4-RR)

    IF Staining of HepG2 using 84668-4-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CEBPA antibody (84668-4-RR, Clone: 242101C9 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of HeLa cells using CEBPA Recombinant antibody (84668-4-RR)

    FC experiment of HeLa using 84668-4-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug CEBPA Recombinant antibody (84668-4-RR, Clone:242101C9) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Flow cytometry (FC) experiment of HepG2 cells using CEBPA Recombinant antibody (84668-4-RR)

    FC experiment of HepG2 using 84668-4-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug CEBPA Recombinant antibody (84668-4-RR, Clone:242101C9) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

Tested Applications

Positive IF/ICC detected inHepG2 cells, HeLa cells
Positive FC (Intra) detected inHeLa cells, HepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:1000-1:4000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84668-4-RR targets CEBPA in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CEBPA fusion protein Ag29947
Full Name CCAAT/enhancer binding protein (C/EBP), alpha
Calculated Molecular Weight 38 kDa
Observed Molecular Weight 40-45 kDa
GenBank Accession NumberNM_004364
Gene Symbol CEBPA
Gene ID (NCBI) 1050
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CEBPA and its isoforms play important roles in lineage determination and gene activation in a variety of cell types by activating transcription from lineage-specific promoters. CEBPA is a DNA-binding protein that recognizes two different motifs: the CCAAT homology common to many promoters and the enhanced core homology common to many enhancers. In hematopoiesis, C/EBPa is a key factor in driving the development of myeloid cells interacting with a variety of factors, including c-Myc, PU.1, and microRNAs. It can also form heterodimers with the related proteins CEBP-beta and CEBP-gamma. The encoded protein has been shown to bind to the promoter and modulate the expression of the gene encoding leptin which plays an important role in body weight homeostasis. CEBPA can interact with CDK2 and CDK4, thereby inhibiting these kinases and causing growth arrest in cultured cells. Several pathways have been implicated as the means by which CEBPA mediates cell cycle arrest and proliferation, including p21, cyclin-dependent kinases and the E2F complex via c-Myc.

Protocols

Product Specific Protocols
IF protocol for CEBPA antibody 84668-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols