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  • Western Blot (WB) analysis of various lysates using INTS6 Recombinant antibody (84610-1-RR)

    WB analysis using 84610-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84610-1-RR (INTS6 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using INTS6 Recombinant antibody (84610-1-RR)

    IF Staining of HeLa using 84610-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using INTS6 antibody (84610-1-RR, Clone: 242099C7 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 84610-1-RR

    Affinity and Kinetic Characterization of 84610-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84610-1-RR against Human INTS6 were performed. The affinity constant is 0.63 nM.

INTS6 Recombinant antibody

INTS6 {ptg:Brand} Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 84610-1-RR
Clone No.242099C7

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

DICE1, DDX26A, DDX26, DBI1, DBI 1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive WB detected inPC-3 cells, DU 145 cells, HuH-7 cells, MCF-7 cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84610-1-RR targets INTS6 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen INTS6 fusion protein Ag36146 Predict reactive species
Full Name integrator complex subunit 6
Calculated Molecular Weight 100 kDa
Observed Molecular Weight130 kDa
GenBank Accession NumberBC039829
Gene Symbol INTS6
Gene ID (NCBI) 26512
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9UL03
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

INTS6 is a component of the Integrator (INT) complex, a complex involved in the small nuclear RNAs (snRNA) U1 and U2 transcription and in their 3'-box-dependent processing.

Protocols

Product Specific Protocols
WB protocol for INTS6 antibody 84610-1-RRDownload protocol
IF protocol for INTS6 antibody 84610-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols