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  • Western Blot (WB) analysis of various lysates using Phospho-CRKL (Tyr207) Recombinant antibody (84590-1-RR)

    WB analysis using 84590-1-RR

    Non-treated NIH/3T3 cells, phosphatase inhibitor treated NIH/3T3 cells and λ phosphatase treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 84590-1-RR (Phospho-CRKL (Tyr207) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin (66009-1-Ig) antibody as a loading control.

  • Flow cytometry (FC) experiment of K-562 cells using Phospho-CRKL (Tyr207) Recombinant antibody (84590-1-RR)

    FC experiment of K-562 using 84590-1-RR

    1X10^6 K-562 cells untreated (dashed lines) or treated with λ phosphatase which intracellularly stained with 0.06 ug Phospho-CRKL (Tyr207) Recombinant antibody (84590-1-RR, Clone:240881B3) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.06 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

Phospho-CRKL (Tyr207) Recombinant antibody

Phospho-CRKL (Tyr207) {ptg:Brand} Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 84590-1-RR
Clone No.240881B3

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, FC (Intra), ELISA

Synonyms

p-CRKL (Tyr207), p-CRKL, CRKL (Tyr207), CrkL (phospho Y207), phospho CRKL (Tyr 207)

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Size/Concentration: 

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Freight/Packing: -

Quantity

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Tested Applications

Positive WB detected inNIH/3T3 cells, λ phosphatase treated NIH/3T3 cells
Positive FC (Intra) detected inλ phosphatase treated K-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.06 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84590-1-RR targets Phospho-CRKL (Tyr207) in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide Predict reactive species
Full Name v-crk sarcoma virus CT10 oncogene homolog (avian)-like
Calculated Molecular Weight 34 kDa
Observed Molecular Weight39 kDa
GenBank Accession NumberBC043500
Gene Symbol CRKL
Gene ID (NCBI) 1399
RRIDAB_3672086
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP46109
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Similar to other adaptor proteins, Crk functions primarily to assemble molecular complexes to distribute signals within the cell. The best studied regulatory mechanism of Crk proteins is the phosphorylation at a tyrosine located in a linker between the N- and C-terminal SH3 domains (Tyr221 in human CrkII, Tyr207 in human CrkL). (PMID: 24675776)

Protocols

Product Specific Protocols
WB protocol for Phospho-CRKL (Tyr207) antibody 84590-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols