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RGNEF Recombinant antibody, PBS Only (Capture)

RGNEF Uni-rAbTM Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241975E4

Cat no : 84570-2-PBS

Synonyms

ARHGEF28, p190-RhoGEF, p190RhoGEF, p190 RhoGEF, ARG28

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RGNEF Recombinant antibody (84570-2-RR)

    WB analysis using 84570-2-RR (same clone as 84570-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84570-2-RR (RGNEF antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84570-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01414-1

    Cytometric bead array standard curve of MP01414-1

    Cytometric bead array standard curve of MP01414-1, RGNEF Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84570-2-PBS. Detection antibody: 84570-1-PBS. Standard: Ag10915. Range: 0.469-60 ng/mL.

Product Information

84570-2-PBS targets RGNEF as part of a matched antibody pair:

MP01414-1: 84570-2-PBS capture and 84570-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen RGNEF fusion protein Ag10915
Full Name Rho-guanine nucleotide exchange factor
Calculated Molecular Weight 192 kDa
Observed Molecular Weight192 kDa
GenBank Accession NumberBC012946
Gene Symbol RGNEF
Gene ID (NCBI) 64283
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

ARHGEF28, also known as RGNEF or p190RhoGEF, is a 190 kDa RNA binding protein (RBP) that contains a Dbl/PH domain capable of RhoA activation. ARHGEF28 interacts with low molecular weight neurofilament mRNA and may be involved in the formation of amyotrophic lateral sclerosis neurofilament aggregates.