RGNEF Recombinant antibody

RGNEF Uni-rAb^TM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

241975E4

Cat no : 84570-2-RR

Synonyms

ARHGEF28, p190-RhoGEF, p190RhoGEF, p190 RhoGEF, ARG28

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Validation Data Gallery

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  WB analysis using 84570-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84570-2-RR (RGNEF antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84570-2-RR is RGNEF Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: RGNEF fusion protein Ag10915
• Full Name: Rho-guanine nucleotide exchange factor
• Calculated Molecular Weight: 192 kDa
• Observed Molecular Weight: 192 kDa
• GenBank Accession Number: BC012946
• Gene Symbol: RGNEF
• Gene ID (NCBI): 64283
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ARHGEF28, also known as RGNEF or p190RhoGEF, is a 190 kDa RNA binding protein (RBP) that contains a Dbl/PH domain capable of RhoA activation. ARHGEF28 interacts with low molecular weight neurofilament mRNA and may be involved in the formation of amyotrophic lateral sclerosis neurofilament aggregates.