Various lysates were subjected to SDS PAGE followed by western blot with 84570-2-RR (RGNEF antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 84570-2-RR (RGNEF antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
Affinity and Kinetic Characterization of 84570-2-RR
Biolayer interferometry (BLl) kinetic assays of 84570-2-RR against Human RGNEF were performed. The affinity constant is below 1 pM.
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
ARHGEF28, also known as RGNEF or p190RhoGEF, is a 190 kDa RNA binding protein (RBP) that contains a Dbl/PH domain capable of RhoA activation. ARHGEF28 interacts with low molecular weight neurofilament mRNA and may be involved in the formation of amyotrophic lateral sclerosis neurofilament aggregates.
Various lysates were subjected to SDS PAGE followed by western blot with 84570-2-RR (RGNEF antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
AFFINITY Figures
Affinity and Kinetic Characterization of 84570-2-RR
Biolayer interferometry (BLl) kinetic assays of 84570-2-RR against Human RGNEF were performed. The affinity constant is below 1 pM.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
RGNEF Recombinant antibody
Catalog Number
84570-2-RR
Citations
-
Dilutions
WB : 1:1000-1:8000
Applications
WB, ELISA
Reactivity
human
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
