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Integrin beta-8 Recombinant antibody

Integrin beta-8 Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

241549A12

Cat no : 84558-1-RR

Synonyms

ITGB8, integrin, beta 8, Integrin beta 8, 241549A12

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Integrin beta-8 Recombinant antibody (84558-1-RR)

    WB analysis using 84558-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84558-1-RR (Integrin beta-8 antibody) at dilution of 1:4500 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84558-1-RR

    Affinity and Kinetic Characterization of 84558-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84558-1-RR against Human Integrin beta-8 were performed. The affinity constant is below 1 pM.

Tested Applications

Positive WB detected inHEK-293 cells, human placenta tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:9000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84558-1-RR is Integrin beta-8 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Integrin beta-8 fusion protein Ag31161
Full Name integrin, beta 8
Calculated Molecular Weight 86 kDa
Observed Molecular Weight95-100 kDa
GenBank Accession NumberNM_002214
Gene Symbol Integrin beta 8
Gene ID (NCBI) 3696
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated alpha and beta subunits. Integrin beta-8 (ITGB8), belongs to the integrin beta chain family, contains an N-terminal signal peptide, a large extracellular domain that includes four cysteine-rich repeats, a transmembrane domain, and a C-terminal cytoplasmic domain (PMID: 1918072). Integrin beta-8 associates with integrin alpha-V (ITGAV) to form an αvβ8 heterodimer which has been shown to bind vitronectin, fibronectin, and a latency-associated peptide of TGF-β1/3 (PMID: 27033701). Integrin beta-8 contains multiple potential N-linked glycosylation sites. The observed molecular mass of approximately 95-100 kDa for integrin beta-8 is larger than the predicted 86 kDa, consistent with substantial glycosylation (PMID: 1918072).

Protocols

Product Specific Protocols
WB protocol for Integrin beta-8 antibody 84558-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols