Filter: AllWBSandwich ELISASample testFC (Intra)Cytometric bead arrayAffinity

WB analysis using 84537-1-RR (same clone as 84537-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 84537-1-RR (RAD51C antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84537-1-PBS in a different storage buffer formulation.
Sandwich ELISA standard curve of MP01399-3
Sandwich ELISA standard curve of MP01399-3, Human RAD51C Recombinant Matched Antibody Pair - PBS only. 84537-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag35070. 84537-1-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL.
Sample test of MP01399-3
The mean RAD51C concentration was determined to be 7.5 ng/mL in MCF-7 cell extract based on a 1.2 mg/mL extract load, 7.7 ng/mL in HeLa cell extract based on a 1.0 mg/mL extract load and 2.7 ng/mL in MDA-MB-231 cell extract based on a 1.9 mg/mL extract load.
FC experiment of MCF-7 using 84537-1-RR (same clone as 84537-1-PBS)
1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug RAD51C Recombinant antibody (84537-1-RR, Clone:241971D4) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84537-1-PBS in a different storage buffer formulation.
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1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug RAD51C Recombinant antibody (84537-1-RR, Clone:241971D4) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84537-1-PBS in a different storage buffer formulation.
Cytometric bead array standard curve of MP01399-1
Cytometric bead array standard curve of MP01399-1, RAD51C Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84537-1-PBS. Detection antibody: 84537-2-PBS. Standard: Ag35070. Range: 0.469-60 ng/mL.
Cytometric bead array standard curve of MP01399-2
Cytometric bead array standard curve of MP01399-2, RAD51C Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84537-1-PBS. Detection antibody: 84537-3-PBS. Standard: Ag35070. Range: 0.469-60 ng/mL.
Affinity and Kinetic Characterization of 84537-1-RR
Biolayer interferometry (BLl) kinetic assays of 84537-1-RR against Human RAD51C were performed. The affinity constant is below 1 pM.

RAD51C Recombinant antibody, PBS Only (Capture/Detector)

RAD51C {ptg:Brand} Recombinant Antibody for WB, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 84537-1-PBS

Clone No.241971D4

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

241971D4

Formulation: PBS Only

PBS and Azide

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

84537-1-PBS targets RAD51C as part of a matched antibody pair:

MP01399-1: 84537-1-PBS capture and 84537-2-PBS detection (validated in Cytometric bead array)

MP01399-2: 84537-1-PBS capture and 84537-3-PBS detection (validated in Cytometric bead array)

MP01399-3: 84537-4-PBS capture and 84537-1-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	RAD51C fusion protein Ag35070 Predict reactive species
Full Name	RAD51 homolog C (S. cerevisiae)
Calculated Molecular Weight	376 aa, 42 kDa
Observed Molecular Weight	42 kDa
GenBank Accession Number	BC107753
Gene Symbol	RAD51C
Gene ID (NCBI)	5889
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	O43502
Storage Buffer	PBS only , pH 7.3
Storage Conditions	Store at -80°C.

Background Information

RAD51C (DNA repair protein RAD51 homolog 3) is essential for the homologous recombination (HR) pathway of DNA repair and is involved in the homologous recombination repair (HRR) pathway of double-stranded DNA breaks arising during DNA replication or induced by DNA-damaging agents (PMID: 14716019; 16215984; 16395335).