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IL-1RA Recombinant antibody

IL-1RA Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

241879B3

Cat no : 84532-4-RR

Synonyms

IL 1ra, IL1RN, IL1RA, IL 1RN, ICIL-1RA

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using IL-1RA Recombinant antibody (84532-4-RR)

    WB analysis using 84532-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84532-4-RR (IL-1RA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84532-4-RR

    Affinity and Kinetic Characterization of 84532-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84532-4-RR against Human IL-1RA were performed. The affinity constant is 0.302 nM.

Tested Applications

Positive WB detected inA431 cells, HepG2 cells, THP-1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84532-4-RR targets IL-1RA in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen IL-1RA fusion protein Ag1277
Full Name interleukin 1 receptor antagonist
Calculated Molecular Weight 20 kDa
Observed Molecular Weight16-18 kDa
GenBank Accession NumberBC009745
Gene Symbol IL-1RA
Gene ID (NCBI) 3557
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Interleukin-1 receptor antagonist (IL-1RA) is a critical member of the IL-1 family of cytokines, which plays a significant role in modulating inflammatory responses. IL-1RA exists in two main forms: a secreted form (sIL-1Ra) and an intracellular form (icIL-1Ra). The secreted form is produced by various cells including monocytes, macrophages, neutrophils, and other cells, while the intracellular form is found in keratinocytes and other epithelial cells, as well as in monocytes and fibroblasts. IL-1RA is important in host defense against endotoxin-induced injury and is produced in numerous experimental animal models of disease, as well as in human autoimmune and chronic inflammatory diseases. It acts as an anti-inflammatory agent by maintaining the balance between pro-inflammatory IL-1α/β and its own anti-inflammatory effects.

Protocols

Product Specific Protocols
WB protocol for IL-1RA antibody 84532-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols