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CKAP4 Recombinant antibody

CKAP4 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

241871B6

Cat no : 84485-4-RR

Synonyms

p63, ERGIC 63, Climp-63, CLIMP 63, 63 kDa membrane protein

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CKAP4 Recombinant antibody (84485-4-RR)

    WB analysis using 84485-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84485-4-RR (CKAP4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CKAP4 Recombinant antibody (84485-4-RR)

    IF Staining of HepG2 using 84485-4-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CKAP4 antibody (84485-4-RR, Clone: 241871B6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 84485-4-RR

    Affinity and Kinetic Characterization of 84485-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84485-4-RR against Human CKAP4 were performed. The affinity constant is 2.71 nM.

Tested Applications

Positive WB detected inA431 cells, HeLa cells, HepG2 cells, A549 cells
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84485-4-RR targets CKAP4 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CKAP4 fusion protein Ag10022
Full Name cytoskeleton-associated protein 4
Calculated Molecular Weight 602 aa, 66 kDa
Observed Molecular Weight 63 kDa
GenBank Accession NumberBC082972
Gene Symbol CKAP4
Gene ID (NCBI) 10970
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CKAP4 (Cytoskeleton-associated protein 4), also known as CLIMP-63 (cytoskeleton-linking membrane protein 63), is a 63 kDa cytoskeleton-linking membrane protein. CKAP4 is a novel DKK1-binding protein on the cell surface membrane of Madin-Darby canine kidney (MDCK) epithelial cells, and evaluated CKAP4-mediated DKK1 signaling in cancer cell proliferation (PMID: 27322059). In addition, CKAP4 was modified with palmitate at Cys100 by palmitoyl acyltransferase DHHC2 (PMID: 18296695), and palmitoylation was required for CKAP4 localization to PM lipid rafts to promote cancer cell proliferation (PMID: 31744930).

Protocols

Product Specific Protocols
WB protocol for CKAP4 antibody 84485-4-RRDownload protocol
IF protocol for CKAP4 antibody 84485-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols