Integrin alpha-5 Recombinant antibody
Integrin alpha-5 Uni-rAbTM Recombinant Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, ELISA
Conjugate
Unconjugated
CloneNo.
241684E8
Cat no : 84468-5-RR
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | A549 cells, HeLa cells, human placenta tissue |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
84468-5-RR targets Integrin alpha-5 in WB, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | Recombinant Protein |
Full Name | integrin, alpha 5 (fibronectin receptor, alpha polypeptide) |
Calculated Molecular Weight | 115 kDa |
Observed Molecular Weight | 140 kDa |
GenBank Accession Number | BC008786 |
Gene Symbol | Integrin alpha 5 |
Gene ID (NCBI) | 3678 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purfication |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated alpha and beta subunits. Integrin alpha 5 (ITGA5), belongs to the integrin alpha chain family, primarily binds to Integrin beta 1 (ITGB1) to form an α5β1 heterodimer (PMID: 33711961). Integrin α5β1 is a specific receptor of fibronectin through its arginine-glycine-aspartic acid (RGD) binding site (PMID: 19608542). Integrin α5β1 plays roles in cell adhesion, migration and matrix formation, and has emerged as an essential mediator in many human carcinomas (PMID: 33408483).
Protocols
Product Specific Protocols | |
---|---|
WB protocol for Integrin alpha-5 antibody 84468-5-RR | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |