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CD40 Recombinant antibody

CD40 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

241760A11

Cat no : 84406-2-RR

Synonyms

p50, CD40L receptor, Bp50, B-cell surface antigen CD40, B cell surface antigen CD40

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CD40/TNFRSF5 Recombinant antibody (84406-2-RR)

    WB analysis using 84406-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84406-2-RR (CD40/TNFRSF5 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of Raji cells using CD40/TNFRSF5 Recombinant antibody (84406-2-RR)

    IF Staining of Raji using 84406-2-RR

    Immunofluorescent analysis of (4% PFA) fixed Raji cells using CD40/TNFRSF5 antibody (84406-2-RR, Clone: 241760A11 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Tested Applications

Positive WB detected inRaji cells, Daudi cells, Ramos cells
Positive IF/ICC detected inRaji cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84406-2-RR targets CD40 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen FusionProtein
Full Name CD40 molecule, TNF receptor superfamily member 5
Calculated Molecular Weight31kDa
Observed Molecular Weight45 kDa
GenBank Accession NumberNM_001250.6
Gene Symbol CD40
Gene ID (NCBI) 958
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cluster of differentiation 40 (CD40) is a costimulatory protein located on antigen presenting cells and is required for their activation. CD40 is a member of the tumor necrosis factor (TNF) receptor (TNFR) family.

What is the molecular weight of CD40?

The molecular weight of CD40 is 43 kDa.

What is the cellular localization of CD40?

CD40 can be secreted by cells or found in the cell membrane.

What is the tissue specificity of CD40?

CD40 is expressed in B cells and primary carcinoma cells but is also found in dendritic cells and macrophages (PMID: 10209159).

What is the function of CD40?

CD40 acts as a receptor for TNFSF5/CD40LG, which is expressed on activated T cells. This interaction is essential for B cell proliferation, expression of activation markers, immunoglobulin production, and isotype switching (PMID: 8809473). This interaction is also crucial for the formation of memory B cells and germinal centers, and signaling through CD40 prevents apoptosis of germinal center B cells.

What is the role of CD40 in disease?

Defects in CD40 lead to hyper-IgM immunodeficiency syndrome type 3 (HIGM3) (PMID: 11675497). This is an autosomal recessive disorder that includes the inability of B cells to undergo isotype switching, a key step in the final differentiation of the humoral immune response, and an inability to mount an antibody-specific immune response.



Protocols

Product Specific Protocols
WB protocol for CD40 antibody 84406-2-RRDownload protocol
IF protocol for CD40 antibody 84406-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols